Analysis of nucleotide insertion opposite urea and translesion synthesis across urea by DNA polymerases-Reference-Cited by-同舟云学术

Analysis of nucleotide insertion opposite urea and translesion synthesis across urea by DNA polymerases

Published:2022-02-15 Issue:1 Volume:44 Page:
ISSN:1880-7062
Container-title:Genes and Environment
language:en
Short-container-title:Genes and Environ

Author:

Kawada Taishu,Kino Katsuhito,Tokorodani Kyousuke,Anabuki Ryuto,Morikawa Masayuki,Kobayashi Takanobu,Ohara Kazuaki,Ohshima Takayuki,Miyazawa Hiroshi

Abstract

Abstract Urea (Ua) is produced in DNA as the result of oxidative damage to thymine and guanine. It was previously reported that Klenow fragment (Kf) exo− incorporated dATP opposite Ua, and that DNA polymerase β was blocked by Ua. We report here the following nucleotide incorporations opposite Ua by various DNA polymerases: DNA polymerase α, dATP and dGTP (dATP > dGTP); DNA polymerase δ, dATP; DNA polymerase ζ, dATP; Kf exo−, dATP; Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4), dGTP and dATP (dGTP > dATP); and DNA polymerase η, dCTP, dGTP, dATP, and dTTP (dCTP > dGTP > dATP > dTTP). DNA polymerases β and ε were blocked by Ua. Elongation by DNA polymerases δ and ζ stopped after inserting dATP opposite Ua. Importantly, the elongation efficiency to full-length beyond Ua using DNA polymerase η and Dpo4 were almost the same as that of natural DNA. Graphical abstract

Funder

JSPS KAKENHI

Publisher

Springer Science and Business Media LLC

Subject

Environmental Science (miscellaneous),Genetics,Social Psychology

Link

https://link.springer.com/content/pdf/10.1186/s41021-022-00236-3.pdf

Reference45 articles.

1. Teoule R, Bert C, Bonicel A. Thymine fragment damage retained in the DNA polynucleotide chain after gamma irradiation in aerated solutions. II Radiat Res. 1977;72(2):190–200. https://doi.org/10.2307/3574690.

2. Jensen WE, Jones AS, Ross GW. Glycosylureas. Part II. The synthesis and properties of 2-deoxy-D-ribosylureas. J Chem Soc. 1965;451:2463–5. https://doi.org/10.1039/jr9650002463.

3. Breimer L, Lindahl T. A DNA glycosylase from Escherichia coli that releases free urea from a polydeoxyribonucleotide containing fragments of base residues. Nucleic Acids Res. 1980;8(24):6199–211. https://doi.org/10.1093/nar/8.24.6199.

4. Ide H, Kow YW, Wallace SS. Thymine glycols and urea residues in M13 DNA constitute replicative blocks in vitro. Nucleic Acids Res. 1985;13(22):8035–52. https://doi.org/10.1093/nar/13.22.8035.

5. Kow YW, Wallace SS. Exonuclease III recognizes urea residues in oxidized DNA. Proc Natl Acad Sci U S A. 1985;82(24):8354–8. https://doi.org/10.1073/pnas.82.24.8354.

Cited by 2 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Replication Bypass of the N-(2-Deoxy-d-erythro-pentofuranosyl)-urea DNA Lesion by Human DNA Polymerase η;Biochemistry;2024-02-27

2. Base excision repair of the N-(2-deoxy-d-erythro-pentofuranosyl)-urea lesion by the hNEIL1 glycosylase;Nucleic Acids Research;2023-04-04