Abstract
Abstract
Background
Colorectal adenocarcinoma (CRA) is one of the leading causes of cancer-related deaths in the world. Long non-coding RNAs (lncRNAs) have been implicated to be effective regulators in the disease course of human cancers, including CRA. Small nucleolar RNA host gene 17 (SNHG17) belongs to lncRNAs, and it has been reported in breast cancer and gastric cancer. However, the function of SNHG17 and its mechanism in CRA progression remain largely unknown. In this study, we attended to shedding some light on the role of SNHG17 in CRA.
Methods
RT-qPCR was used to assess SNHG17 expression in CRA cells. CCK-8 assay, colony formation and transwell assay were carried out to detect the regulatory effect of SNHG17 silencing on CRA cell proliferation and migration. The angiogenesis of SNHG7-downregulated CRA cells was analyzed by tube formation assay. Mechanism experiments were conducted to identify the interaction between miR-23a-3p and SNHG17 or C-X-C motif chemokine ligand 12 (CXCL12).
Results
SNHG17 possessed with high expression in CRA cells. Knockdown of SNHG17 caused the inhibition on CRA cell proliferation and migration. SNHG17 promoted CRA cell proliferation and migration by sponging miR-23a-3p to upregulate CXCL12.
Conclusion
SNHG17 promotes the proliferation and migration of CRA cells by inhibiting miR-23a-3p to modulate CXCL12-mediated angiogenesis.
Funder
China Postdoctoral Science Foundation
the National Natural Science Foundation of China
Project Natural Science Foundation of Guizhou Province
the Top Talent Foundation of the Department of Education of Guizhou Province
the Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences
Project Natural Science Foundation of Guiyang City
Doctoral Foundation of the First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine
Project Foundation of Guizhou Health Committee
Project Foundation of Guizhou Administration of Traditional Chinese Medicine
Publisher
Springer Science and Business Media LLC
Subject
Cancer Research,Genetics,Oncology
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