Author:
Wang Jianfei,Huang Jiaqi,Laffend Hannah,Jiang Shaoming,Zhang Jing,Ning Yuchen,Fang Mudannan,Liu Shijie
Abstract
AbstractParametric optimization is an effective way in fermentation process to improve product yield and productivity in order to save time, space and financial resources. In this study, Box–Behnken design was applied to optimize the conditions for lactic acid production by immobilized Lactobacillus pentosus ATCC 8041 cell fermentation. Two quadratic models and response surface methodology were performed to illustrate the effect of each parameters and their interactions on the lactic acid yield and glucose consumption rate in immobilized L. pentosus ATCC 8041 cell fermentation. The maximum lactic acid yield was obtained as 0.938 ± 0.003 g/g glucose with a productivity of 2.213 ± 0.008 g/(L × h) under the optimized conditions of 2.0 mm bead diameter, 5.60 pH, 115.3 g/L initial glucose concentration, and 398.2 mg biomass (CDW) in 100 mL hydrogel. The analysis of variance indicated that the quadratic model was significant and could be used to scale up the fermentation process.
Publisher
Springer Science and Business Media LLC
Subject
Renewable Energy, Sustainability and the Environment,Biomedical Engineering,Food Science,Biotechnology
Reference62 articles.
1. Abdel-Rahman MA, Tashiro Y, Sonomoto K (2011) Lactic acid production from lignocellulose-derived sugars using lactic acid bacteria: overview and limits. J Biotechnol 156(4):286–301
2. Al-Gheethi A, Noman E, Mohamed RMSR, Ismail N, Abdullah AHB, Kassim AHM (2019) Optimizing of pharmaceutical active compounds biodegradability in secondary effluents by β-lactamase from Bacillus subtilis using central composite design. J Hazard Mater 365:883–894
3. Aslan N, Cebeci Y (2007) Application of Box–Behnken design and response surface methodology for modeling of some Turkish coals. Fuel 86(1–2):90–97
4. Bahry H, Abdalla R, Pons A, Taha S, Vial C (2019) Optimization of lactic acid production using immobilized Lactobacillus rhamnosus and carob pod waste from the Lebanese food industry. J Biotechnol 306:81–88
5. Bhatnagar Y, Singh GB, Mathur A, Srivastava S, Gupta S, Gupta N (2016) Biodegradation of carbazole by Pseudomonas sp. GBS. 5 immobilized in polyvinyl alcohol beads. J Biochem Technol 6(3):1003–1007
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