Downsizing a pullulanase to a small molecule with improved soluble expression and secretion efficiency in Escherichia coli-Reference-Cited by-同舟云学术

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Downsizing a pullulanase to a small molecule with improved soluble expression and secretion efficiency in Escherichia coli

Published:2016-01-14 Issue:1 Volume:15 Page:
ISSN:1475-2859
Container-title:Microbial Cell Factories
language:en
Short-container-title:Microb Cell Fact

Author:

Chen Ana,Sun Yang,Zhang Wei,Peng Feng,Zhan Chunjun,Liu Meng,Yang Yankun,Bai Zhonghu

Funder

National Basic Research Program of China (973 Program)

Fundamental Research Funds for the Central Universities

Publisher

Springer Science and Business Media LLC

Subject

Applied Microbiology and Biotechnology,Bioengineering,Biotechnology

Link

http://link.springer.com/content/pdf/10.1186/s12934-015-0403-5

Reference34 articles.

1. Singh RS, Saini GK, Kennedy JF. Continuous hydrolysis of pullulan using covalently immobilized pullulanase in a packed bed reactor. Carbohydr Polym. 2011;83:672–5.

2. Nie Y, Yan W, Xu Y, Chen WB, Mu XQ, Wang X, et al. High-level expression of Bacillus naganoensis pullulanase from recombinant Escherichia coli with auto-induction: effect of lac operator. PLoS One. 2013;8:e78416.

3. Talekar S, Pandharbale A, Ladole M, Nadar S, Mulla M, Japhalekar K, et al. Carrier free co-immobilization of alpha amylase, glucoamylase and pullulanase as combined cross-linked enzyme aggregates (combi-CLEAs): a tri-enzyme biocatalyst with one pot starch hydrolytic activity. Bioresour Technol. 2013;147:269–75.

4. Roy I, Gupta MN. Hydrolysis of starch by a mixture of glucoamylase and pullulanase entrapped individually in calcium alginate beads. Enzyme and Microbial Technology. 2004;34:26–32.

5. Hii SL, Tan JS, Ling TC, Ariff AB. Pullulanase: role in starch hydrolysis and potential industrial applications. Enzyme Res. 2012;2012:921362.

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