The visible touch: in planta visualization of protein-protein interactions by fluorophore-based methods
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Published:2006-06-26
Issue:1
Volume:2
Page:
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ISSN:1746-4811
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Container-title:Plant Methods
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language:en
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Short-container-title:Plant Methods
Author:
Bhat Riyaz A,Lahaye Thomas,Panstruga Ralph
Abstract
Abstract
Non-invasive fluorophore-based protein interaction assays like fluorescence resonance energy transfer (FRET) and bimolecular fluorescence complementation (BiFC, also referred to as "split YFP") have been proven invaluable tools to study protein-protein interactions in living cells. Both methods are now frequently used in the plant sciences and are likely to develop into standard techniques for the identification, verification and in-depth analysis of polypeptide interactions. In this review, we address the individual strengths and weaknesses of both approaches and provide an outlook about new directions and possible future developments for both techniques.
Publisher
Springer Science and Business Media LLC
Subject
Plant Science,Genetics,Biotechnology
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