Author:
Nagarajha Selvan Lakshmi Dhevi,Kaviyil Jyothi Embekkat,Nirujogi Raja Sekhar,Muthusamy Babylakshmi,Puttamallesh Vinuth N,Subbannayya Tejaswini,Syed Nazia,Radhakrishnan Aneesha,Kelkar Dhanashree S,Ahmad Sartaj,Pinto Sneha M,Kumar Praveen,Madugundu Anil K,Nair Bipin,Chatterjee Aditi,Pandey Akhilesh,Ravikumar Raju,Gowda Harsha,Prasad Thottethodi Subrahmanya Keshava
Abstract
Abstract
Background
Cryptococcus neoformans, a basidiomycetous fungus of universal occurrence, is a significant opportunistic human pathogen causing meningitis. Owing to an increase in the number of immunosuppressed individuals along with emergence of drug-resistant strains, C. neoformans is gaining importance as a pathogen. Although, whole genome sequencing of three varieties of C. neoformans has been completed recently, no global proteomic studies have yet been reported.
Results
We performed a comprehensive proteomic analysis of C. neoformans var. grubii (Serotype A), which is the most virulent variety, in order to provide protein-level evidence for computationally predicted gene models and to refine the existing annotations. We confirmed the protein-coding potential of 3,674 genes from a total of 6,980 predicted protein-coding genes. We also identified 4 novel genes and corrected 104 predicted gene models. In addition, our studies led to the correction of translational start site, splice junctions and reading frame used for translation in a number of proteins. Finally, we validated a subset of our novel findings by RT-PCR and sequencing.
Conclusions
Proteogenomic investigation described here facilitated the validation and refinement of computationally derived gene models in the intron-rich genome of C. neoformans, an important fungal pathogen in humans.
Publisher
Springer Science and Business Media LLC
Subject
Clinical Biochemistry,Molecular Biology,Molecular Medicine,Clinical Biochemistry,Molecular Biology,Molecular Medicine
Cited by
18 articles.
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