Characterization and optimization of extracellular enzymes production by Aspergillus niger strains isolated from date by-products

Author:

Bellaouchi RedaORCID,Abouloifa Houssam,Rokni Yahya,Hasnaoui Amina,Ghabbour Nabil,Hakkou Abdelkader,Bechchari Abdelmajid,Asehraou Abdeslam

Abstract

Abstract Background This work aims to study the optimal conditions of the fermentation culture medium used for the production of extracellular enzymes (amylase, cellulase, lipase, and protease) from previously isolated Aspergillus niger strains in date by-products. Results The five most powerful isolates selected based on the zone of degradation formed on Petri plates by the substrate were subjected to the quantitative evaluation of their enzymatic production. All five strains showed almost similar API-ZYM profiles, with minor variations observed at the level of some specific enzyme expression. The production of cellulase and amylase was depending on pH and incubation temperatures. ASP2 strain demonstrated the high production rate of amylase (at pH 5 and 30 °C) and cellulase (at pH 6 and 30 °C) for 96 h of incubation. Conclusion The A. niger showed the ability to produce several extracellular enzymes and can be used in the valorization of different agroindustrial residues.

Publisher

Springer Science and Business Media LLC

Subject

Genetics,Biotechnology

Reference28 articles.

1. Berka RM, Dunn-Coleman N, Ward M (1992) Industrial enzymes from Aspergillus species. Biotechnol 23:155

2. Pandey A, Selvakumar P, Soccol CR, Poonam Nigam (1999) Solid-state fermentation for the production of industrial enzymes. Curr Sci:149–162

3. Coutinho PM, Andersen MR, Kolenova K, vanKuyk PA, Benoit I, Gruben BS, Trejo-Aguilar B, Visser H, van Solingen P, Pakula T (2009) Post-genomic insights into the plant polysaccharide degradation potential of Aspergillus nidulans and comparison to Aspergillus niger and Aspergillus oryzae. Fungal Genet Biol 46(1):S161–S169. https://doi.org/10.1016/j.fgb.2008.07.020

4. de Vries RP, Visser J (2001) Aspergillus enzymes involved in degradation of plant cell wall polysaccharides. Microbiol Mol Biol Rev 65(4):497–522. https://doi.org/10.1128/MMBR.65.4.497-522.2001

5. Bellaouchi R, Ghomari I, Hasnaoui A, Hakkou A, Bechchari A, Chihib NE, Asehraou A (2017) Physico-chemical and microbial properties of undervalued dates and processed dates by-products in Morocco. Int Food Re J 24:963–969

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