Characterization and remediation of sample index swaps by non-redundant dual indexing on massively parallel sequencing platforms

Author:

Costello MauraORCID,Fleharty Mark,Abreu Justin,Farjoun Yossi,Ferriera Steven,Holmes Laurie,Granger Brian,Green Lisa,Howd Tom,Mason Tamara,Vicente Gina,Dasilva Michael,Brodeur Wendy,DeSmet Timothy,Dodge Sheila,Lennon Niall J.,Gabriel Stacey

Funder

Center for Common Disease

Publisher

Springer Science and Business Media LLC

Subject

Genetics,Biotechnology

Reference18 articles.

1. Shen MR, Boutell JM, Stephens KM, Ronaghi M, Gunderson K, Venkatesan BM, Bowen MS, Vijayan K. Kinetic exclusion amplification of nucleic acid libraries. USPTO 20160053310:A1. US Patent , filed October 9, 2015, and issued February 25, 2016.

2. Illumina, Inc. Illumina HiSeqX series specification sheet. 2017. https://www.illumina.com/content/dam/illumina-marketing/documents/products/datasheets/datasheet-hiseq-x-ten.pdf . Accessed 15 Nov 2017.

3. Illumina, Inc. Illumina NovaSeq specification sheet. 2017. https://www.illumina.com/content/dam/illumina-marketing/documents/products/datasheets/novaseq-6000-system-specification-sheet-770-2016-025.pdf . Accessed 15 Nov 2017.

4. Sinha R, Stanley G, Gulati GS, Ezran C, Travaglini KJ, Wei E, et al. Index switching causes ‘spreading-of-signal’ among multiplexed samples in illumina HiSeq 4000 DNA sequencing. bioRxiv. 2017; https://doi.org/10.1101/125724 .

5. Illumina, Inc. Effects of index Misassignment on multiplexing and downstream analysis. 2017. https://www.illumina.com/content/dam/illumina-marketing/documents/products/whitepapers/index-hopping-white-paper-770-2017-004.pdf?linkId=36607862 . Accessed 15 Nov 2017.

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