Integrative analysis of transcriptomics and metabolomics to reveal the melanogenesis pathway of muscle and related meat characters in Wuliangshan black-boned chickens

Author:

Dou Tengfei,Yan Shixiong,Liu Lixian,Wang Kun,Jian Zonghui,Xu Zhiqiang,Zhao Jingying,Wang Qiuting,Sun Shuai,Talpur Mir Zulqarnain,Duan Xiaohua,Gu Dahai,He Yang,Du Yanli,Abdulwahid Alsoufi Mohammed,Li Qihua,Rong Hua,Cao Weina,Su Zhengchang,Zhao Guiping,Liu Ranran,Zhao Sumei,Huang Ying,te Pas Marinus F. W.,Ge Changrong,Jia Junjing

Abstract

Abstract Background Melanin is an important antioxidant in food and has been used in medicine and cosmetology. Chicken meat with high melanin content from black-boned chickens have been considered a high nutritious food with potential medicinal properties. The molecular mechanism of melanogenesis of skeletal muscle in black-boned chickens remain poorly understood. This study investigated the biological gene-metabolite associations regulating the muscle melanogenesis pathways in Wuliangshan black-boned chickens with two normal boned chicken breeds as control. Results We identified 25 differentially expressed genes and 11 transcription factors in the melanogenesis pathways. High levels of the meat flavor compounds inosine monophosphate, hypoxanthine, lysophospholipid, hydroxyoctadecadienoic acid, and nicotinamide mononucleotide were found in Wuliangshan black-boned chickens. Conclusion Integrative analysis of transcriptomics and metabolomics revealed the dual physiological functions of the PDZK1 gene, involved in pigmentation and/or melanogenesis and regulating the phospholipid signaling processes in muscle of black boned chickens.

Publisher

Springer Science and Business Media LLC

Subject

Genetics,Biotechnology

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