Abstract
AbstractTable-top extreme ultraviolet (EUV) microscopy offers unique opportunities for label-free investigation of biological samples. Here, we demonstrate ptychographic EUV imaging of two dried, unstained model specimens: germlings of a fungus (Aspergillus nidulans), and bacteria (Escherichia coli) cells at 13.5 nm wavelength. We find that the EUV spectral region, which to date has not received much attention for biological imaging, offers sufficient penetration depths for the identification of intracellular features. By implementing a position-correlated ptychography approach, we demonstrate a millimeter-squared field of view enabled by infrared illumination combined with sub-60 nm spatial resolution achieved with EUV illumination on selected regions of interest. The strong element contrast at 13.5 nm wavelength enables the identification of the nanoscale material composition inside the specimens. Our work will advance and facilitate EUV imaging applications and enable further possibilities in life science.
Funder
strategy and Innovation Grant from the Freestate of Thuringia
Innovation Pool of the Research Field Matter of the Helmholtz Association of German Research Centers
Leibniz Research Cluster InfectoOptics
Thüringer Ministerium für Bildung, Wissenschaft und Kultur
GSI Helmholtzzentrum für Schwerionenforschung
Fraunhofer-Gesellschaft
Deutsche Forschungsgemeinschaft
Publisher
Springer Science and Business Media LLC
Subject
Atomic and Molecular Physics, and Optics,Electrical and Electronic Engineering,Engineering (miscellaneous)
Cited by
8 articles.
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