A defined road to tracheal reconstruction: laser structuring and cell support for rapid clinic translation
-
Published:2022-07-16
Issue:1
Volume:13
Page:
-
ISSN:1757-6512
-
Container-title:Stem Cell Research & Therapy
-
language:en
-
Short-container-title:Stem Cell Res Ther
Author:
Fayzullin Alexey, Vladimirov Georgiy, Kuryanova Anastasia, Gafarova Elvira, Tkachev Sergei, Kosheleva Nastasia, Istranova Elena, Istranov Leonid, Efremov Yuri, Novikov Ivan, Bikmulina Polina, Puzakov Kirill, Petrov Pavel, Vyazankin Ivan, Nedorubov Andrey, Khlebnikova Tatyana, Kapustina Valentina, Trubnikov Pavel, Minaev Nikita, Kurkov Aleksandr, Royuk Valery, Mikhailov Vasily, Parshin Dmitriy, Solovieva Anna, Lipina Marina, Lychagin Alexey, Timashev PeterORCID, Svistunov Andrey, Fomin Victor, Shpichka Anastasia
Abstract
AbstractOne of the severe complications occurring because of the patient’s intubation is tracheal stenosis. Its incidence has significantly risen because of the COVID-19 pandemic and tends only to increase. Here, we propose an alternative to the donor trachea and synthetic prostheses—the tracheal equivalent. To form it, we applied the donor trachea samples, which were decellularized, cross-linked, and treated with laser to make wells on their surface, and inoculated them with human gingiva-derived mesenchymal stromal cells. The fabricated construct was assessed in vivo using nude (immunodeficient), immunosuppressed, and normal mice and rabbits. In comparison with the matrix ones, the tracheal equivalent samples demonstrated the thinning of the capsule, the significant vessel ingrowth into surrounding tissues, and the increase in the submucosa resorption. The developed construct was shown to be highly biocompatible and efficient in trachea restoration. These results can facilitate its clinical translation and be a base to design clinical trials.
Graphical Abstract
Funder
Russian Science Foundation
Publisher
Springer Science and Business Media LLC
Subject
Cell Biology,Biochemistry, Genetics and Molecular Biology (miscellaneous),Molecular Medicine,Medicine (miscellaneous)
Reference50 articles.
1. Sarper A, Ayten A, Eser I, Ozbudak O, Demircan A. Tracheal stenosis after tracheostomy or intubation: review with special regard to cause and management. Texas Hear Inst J. 2005;32:154–8. 2. Mattioli F, Marchioni A, Andreani A, Cappiello G, Fermi M, Presutti L. Post-intubation tracheal stenosis in COVID-19 patients. Eur Arch Oto-Rhino-Laryngology. 2021;278:847–8. https://doi.org/10.1007/s00405-020-06394-w. 3. Gervasio CF, Averono G, Robiolio L, Bertoletti M, Colageo U, De Col L, Bertone F. Tracheal stenosis after tracheostomy for mechanical ventilation in COVID-19 pneumonia–a report of 2 cases from Northern Italy. Am J Case Rep. 2020;21:1–5. https://doi.org/10.12659/AJCR.926731. 4. Alturk A, Bara A, Darwish B. Post-intubation tracheal stenosis after severe COVID-19 infection: a report of two cases. Ann Med Surg. 2021;67: 102468. https://doi.org/10.1016/j.amsu.2021.102468. 5. Miwa M, Nakajima M, Kaszynski RH, Hamada S, Nakano T, Shirokawa M, Goto H, Yamaguchi Y. Two cases of post-intubation laryngotracheal stenosis occurring after severe covid-19. Intern Med. 2021;60:473–7. https://doi.org/10.2169/INTERNALMEDICINE.6105-20.
Cited by
5 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|