Abstract
Abstract
Background
Tuber shape and specific gravity (dry matter) are important agronomic traits in potato processing and impact production costs, quality, and consistency of the final processed food products such as French fries and potato chips. In this study, linkage and QTL mapping were performed for these two traits to allow for the implementation of marker-assisted selection to facilitate breeding efforts in the russet market class. Two parents, Rio Grande Russet (female) and Premier Russet (male) and their 205 F1 progenies were initially phenotyped for tuber shape and specific gravity in field trials conducted in Idaho and North Carolina in 2010 and 2011, with specific gravity also being measured in Minnesota in 2011. Progenies and parents were previously genotyped using the Illumina SolCAP Infinium 8303 Potato SNP array, with ClusterCall and MAPpoly (R-packages) subsequently used for autotetraploid SNP calling and linkage mapping in this study. The 12 complete linkage groups and phenotypic data were then imported into QTLpoly, an R-package designed for polyploid QTL analyses.
Results
Significant QTL for tuber shape were detected on chromosomes 4, 7, and 10, with heritability estimates ranging from 0.09 to 0.36. Significant tuber shape QTL on chromosomes 4 and 7 were specific to Idaho and North Carolina environments, respectively, whereas the QTL on chromosome 10 was significant regardless of growing environment. Single marker analyses identified alleles in the parents associated with QTL on chromosomes 4, 7, and 10 that contributed to significant differences in tuber shape among progenies. Significant QTL were also identified for specific gravity on chromosomes 1 and 5 with heritability ranging from 0.12 to 0.21 and were reflected across environments.
Conclusion
Fully automated linkage mapping and QTL analysis were conducted to identify significant QTL for tuber shape and dry matter in a tetraploid mapping population representing the russet market class. The findings are important for the development of molecular markers useful to potato breeders for marker-assisted selection for the long tuber shape and acceptable dry matter required by the potato industry within this important market class.
Funder
National Institute of Food and Agriculture
Publisher
Springer Science and Business Media LLC
Reference56 articles.
1. Suttle J. Symposium Introduction: Enhancing the Nutritional Value of Potato Tubers. American Journal of Potato Research. 2008;85:266.
2. USDA, National Agricultural Statistics Service: Potatoes 2019 Summary. https://usda.library.cornell.edu/concern/publications/fx719m44h (2020). Accessed 02 Dec 2020.
3. Stark JC, Love SL, Knowles NR. Tuber quality. In: Stark J, Thornton M, Nolte P, editors. Potato production systems. 2nd ed. Cham: Springer Nature Switzerland AG; 2020. p. 479–97.
4. Chen N, Zhu W, Xu J, Duan S, Bian C, Hu J, et al. Molecular marker development and primary physical map construction for the tuber shape Ro gene locus in diploid potato (Solanum tuberosum L.). Mol Breed. 2019;39:6.
5. Vreugdenhil D, Bradshaw J, Gebhardt C, Govers F, Taylor MA, MacKerron DK, et al. Potato biology and biotechnology: advances and perspectives: advances and perspectives. Amsterdam: Elsevier; 2011. p. 211–3.
Cited by
7 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献