Author:
Yang Xingai,Cai Jie,Xue Jingjing,Luo Xiuqin,Zhu Wenli,Xiao Xinhui,Xue Maofu,An Feifei,Li Kaimian,Chen Songbi
Abstract
Abstract
Background
Magnesium chelatase plays an important role in photosynthesis, but only a few subunits have been functionally characterized in cassava.
Results
Herein, MeChlD was successfully cloned and characterized. MeChlD encodes a magnesium chelatase subunit D, which has ATPase and vWA conservative domains. MeChlD was highly expressed in the leaves. Subcellular localization suggested that MeChlD:GFP was a chloroplast-localized protein. Furthermore, the yeast two-hybrid system and BiFC analysis indicated that MeChlD interacts with MeChlM and MePrxQ, respectively. VIGS-induce silencing of MeChlD resulted in significantly decreased chlorophyll content and reduction the expression of photosynthesis-related nuclear genes. Furthermore, the storage root numbers, fresh weight and the total starch content in cassava storage roots of VIGS-MeChlD plants was significantly reduced.
Conclusion
Taken together, MeChlD located at the chloroplast is not only required for chlorophyll biosynthesis and photosynthesis, but also affecting the starch accumulation in cassava. This study expands our understanding of the biological functions of ChlD proteins.
Publisher
Springer Science and Business Media LLC
Cited by
1 articles.
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