Author:
Zhou Jing,Chen Siqi,Shi Wenjiao,David-Schwartz Rakefet,Li Sutao,Yang Fulin,Lin Zhanxi
Abstract
AbstractBackgroundGiant Juncao is often used as feed for livestock because of its huge biomass. However, drought stress reduces forage production by affecting the normal growth and development of plants. Therefore, investigating the molecular mechanisms of drought tolerance will provide important information for the improvement of drought tolerance in this grass.ResultsA total of 144.96 Gb of clean data was generated and assembled into 144,806 transcripts and 93,907 unigenes. After 7 and 14 days of drought stress, a total of 16,726 and 46,492 differentially expressed genes (DEGs) were observed, respectively. Compared with normal irrigation, 16,247, 23,503, and 11,598 DEGs were observed in 1, 5, and 9 days following rehydration, respectively. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses revealed abiotic stress-responsive genes and pathways related to catalytic activity, methyltransferase activity, transferase activity, and superoxide metabolic process. We also identified transcription factors belonging to several families, including basic helix-loop-helix (bHLH), WRKY, NAM (no apical meristem), ATAF1/2 and CUC2 (cup-shaped cotyledon) (NAC), fatty acyl-CoA reductase (FAR1), B3, myeloblastosis (MYB)-related, and basic leucine zipper (bZIP) families, which are important drought-rehydration-responsive proteins. Weighted gene co-expression network analysis was also used to analyze the RNA-seq data to predict the interrelationship between genes. Twenty modules were obtained, and four of these modules may be involved in photosynthesis and plant hormone signal transduction that respond to drought and rehydration conditions.ConclusionsOur research is the first to provide a more comprehensive understanding of DEGs involved in drought stress at the transcriptome level in Giant Juncao with different drought and recovery conditions. These results may reveal insights into the molecular mechanisms of drought tolerance in Giant Juncao and provide diverse genetic resources involved in drought tolerance research.
Funder
Natural Science Foundation of Fujian Province
Education department of Fujian Province
National Natural Science Foundation of China
Publisher
Springer Science and Business Media LLC
Reference77 articles.
1. Luo LJ, Xia H, Lu BR. Editorial: crop breeding for drought resistance. Front Plant Sci. 2019;10:314.
2. Banks JM, Percival GC, Rose G. Variations in seasonal drought tolerance rankings. Trees. 2019;33(4):1063–72.
3. Pflug EE, Buchmann N, Siegwolf RTW, Schaub M, Rigling A, Arend M. Resilient leaf physiological response of european beech (Fagus sylvatica L.) to summer drought and drought release. Front. Plant Sci. 2018;9:187.
4. Hein JA, Sherrard ME, Manfredi KP, Abebe T. The fifth leaf and spike organs of barley (Hordeum vulgare L.) display different physiological and metabolic responses to drought stress. BMC Plant Biol. 2016;16(1):248.
5. Torres-Franklin ML, Gigon A, Melo DFD, Zuily-Fodil Y, Pham-Thi AT. Drought stress and rehydration affect the balance between MGDG and DGDG synthesis in cowpea leaves. Physiol Plant. 2007;131(2):201–10.