Author:
Mercado-Curiel Ricardo F,Esquinca-Avilés Héctor Armando,Tovar Rosalinda,Díaz-Badillo Álvaro,Camacho-Nuez Minerva,Muñoz María de Lourdes
Abstract
Abstract
Background
Dengue viruses (DENV) attach to the host cell surface and subsequently enter the cell by receptor-mediated endocytosis. Several primary and low affinity co-receptors for this flavivirus have been identified. However, the presence of these binding molecules on the cell surface does not necessarily render the cell susceptible to infection. Determination of which of them serve as bona fide receptors for this virus in the vector may be relevant to treating DENV infection and in designing control strategies.
Results
(1) Overlay protein binding assay showed two proteins with molecular masses of 80 and 67 kDa (R80 and R67). (2) Specific antibodies against these two proteins inhibited cell binding and infection. (3) Both proteins were bound by all four serotypes of dengue virus. (4) R80 and R67 were purified by affinity chromatography from Ae. aegypti mosquito midguts and from Ae albopictus C6/36 cells. (5) In addition, a protein with molecular mass of 57 kDa was purified by affinity chromatography from the midgut extracts. (6) R80 and R67 from radiolabeled surface membrane proteins of C6/36 cells were immunoprecipitated by antibodies against Ae. aegypti midgut.
Conclusion
Our results strongly suggest that R67 and R80 are receptors for the four serotypes of dengue virus in the midgut cells of Ae. aegypti and in C6/36 Ae. albopictus cells.
Publisher
Springer Science and Business Media LLC
Subject
Microbiology (medical),Microbiology
Reference45 articles.
1. WHO (World Health Organization): Dengue Haemorrhagic fever: early recognition, diagnosis and hospital management – an audiovisual guide for health care workers responding to outbreaks. Weekly Epidemiological Record. 2006, 81 (38): 362-363.
2. DEGPI (Dirección General de Epidemiología de la Secretaría de Salud) 2004. Boletín Epidemiología.
3. Halstead SB: Antibody, macrophages, dengue virus infection, shock, and hemorrhage a pathogenetic cascade. Rev Infect Dis. 1989, 11 (Suppl 4): S830-S839.
4. Littaua R, Kurane I, Ennis FA: Human IgG Fc receptor II mediates antibody-dependent enhancement of dengue virus infection. J Immunol. 1990, 144 (8): 3183-3186.
5. Tu WC, Chen CC, Hou RF: Ultrastructural studies on the reproductive system of male A. aegypti (Diptera: Culicidae) infected with dengue 2 virus. J Medx Entomol. 1998, 35 (1): 71-76.
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