Author:
Hemmerlein Bernhard,Weseloh Rüdiger M,Mello de Queiroz Fernanda,Knötgen Hendrik,Sánchez Araceli,Rubio María E,Martin Sabine,Schliephacke Tessa,Jenke Marc,Heinz-Joachim-Radzun ,Stühmer Walter,Pardo Luis A
Abstract
Abstract
Background
Certain types of potassium channels (known as Eag1, KCNH1, Kv10.1) are associated with the production of tumours in patients and in animals. We have now studied the expression pattern of the Eag1 channel in a large range of normal and tumour tissues from different collections utilising molecular biological and immunohistochemical techniques.
Results
The use of reverse transcription real-time PCR and specifically generated monoclonal anti-Eag1 antibodies showed that expression of the channel is normally limited to specific areas of the brain and to restricted cell populations throughout the body. Tumour samples, however, showed a significant overexpression of the channel with high frequency (up to 80% depending on the tissue source) regardless of the detection method (staining with either one of the antibodies, or detection of Eag1 RNA).
Conclusion
Inhibition of Eag1 expression in tumour cell lines reduced cell proliferation. Eag1 may therefore represent a promising target for the tailored treatment of human tumours. Furthermore, as normal cells expressing Eag1 are either protected by the blood-brain barrier or represent the terminal stage of normal differentiation, Eag1 based therapies could produce only minor side effects.
Publisher
Springer Science and Business Media LLC
Subject
Cancer Research,Oncology,Molecular Medicine
Reference53 articles.
1. Hille B: Ion channels of excitable membranes, Third Edition. 2001, Sunderland, Massachussets , Sinauer Associates Inc..
2. Pardo LA, Contreras-Jurado C, Zientkowska M, Alves F, Stuhmer W: Role of voltage-gated potassium channels in cancer. J Membr Biol. 2005, 205 (3): 115-124. 10.1007/s00232-005-0776-1
3. Pardo LA: Voltage-gated Potassium Channels in Cell Proliferation. Physiology. 2004, 19: 285-292. 10.1152/physiol.00011.2004
4. Stühmer W, Alves F, Hartung F, Zientkowska M, Pardo LA: Potassium channels as tumour markers. FEBS Lett. 2006, 580 (12): 2850-2852. 10.1016/j.febslet.2006.03.062
5. Conti M: Targeting K+ channels for cancer therapy. J Exp Ther Oncol. 2004, 4: 161-166.
Cited by
219 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献