Author:
Cao Liu,Chen Pan,Hou Xingrong,Ma Jun,Yang Ning,Lu Yan,Huang Hai
Abstract
Abstract
Background
Hybridization is a useful strategy to produce offspring with more desirable phenotypic characteristics than those of parents. The hybrid grouper derived from the cross of Cromileptes altivelis (♀, 2n = 48) with Epinephelus lanceolatus (♂, 2n = 48) exhibits improved growth compared with its female parent, which makes it valuable to aquaculture. However, the genetic traits of the hybrid grouper are poorly understood.
Results
The observations showed that the hybrid grouper was diploid (2n = 48) and displayed intermediate morphology with the parent's measurable characteristics. The ribosomal DNA (rDNA) and mitochondria DNA (mtDNA) were characterized at molecular and phylogenetic level. High similarity and low genetic distance of 5S rDNA and mtDNA sequences between the hybrid grouper and C. altivelis showed that the hybrid grouper had a closer genetic relationship with female parents. The reconstructed phylogenetic tree based on COI gene and D-loop region of mtDNA recovered that mtDNA was maternally inherited in the hybrid grouper. Additionally, the DNA methylation level of 5S rDNA intergenic spacers (IGS) sequence was tested in here. The results showed that the DNA methylation status of the hybrid grouper was significantly lower than that of C. altivelis.
Conclusion
Results of this study provide important data on the genetic characteristics of the hybrid derived from the cross of C. altivelis and E. lanceolatus, and contribute the knowledge of both evolution and marine fish breeding.
Funder
The Innovation Platform for Academicians of Hainan Province
Scientific Research Foundation of Hainan Tropical Ocean University
Hainan Provincial Natural Science Foundation of China
National Natural Science Foundation of China
The Major Science and Technology plan of Hainan Province
State Key Laboratory of Developmental Biology of Freshwater Fish
Publisher
Springer Science and Business Media LLC
Subject
Health Informatics,Genetics
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