Author:
Bekele Tizeta,Zewde Girma,Tefera Genene,Feleke Aklilu,Zerom Kaleab
Abstract
Abstract
Background
Although raw meat and its products are commonly consumed in traditional Ethiopian diets, E. coli O157: H7 is rarely studied compared to other countries. Thus the present study has been designed to determine the prevalence and assess the antimicrobial susceptibility of E. coli O157: H7 isolated from beef, sheep meat and goat meat at one abattoir and in 48 selected raw meat retail shops in Addis Ababa.
Results
Out of 384 meat samples examined, 10.2% (39/384) were positive to E. coli O157:H7. Among these samples examined, beef was the most frequently contaminated with E. coli O157:H7 with an overall prevalence of 13.3% (17/128) followed by 9.4% (12/128) sheep meat and 7.8% (10/128) goat meat. With regard to meat source, the prevalence rates of E. coli O157:H7 at the abattoir and the selected retail shops were 5.7% (11/192) and 14.6% (28/192), respectively. Significant differences in prevalence was observed among sample sources (p < 0.05). The antimicrobial susceptibility investigation of 39 E. coli O157:H7 isolates using 10 commercially available antimicrobial discs revealed that the isolates were susceptible to nine antimicrobials from 69.3% to 100% except streptomycin which showed susceptibility of 48.7%. An overall resistance of 33.4% and 30.9% was recorded to streptomycin and amikacin, whereas 5.1%, 5.1%, 7.7%, 12.8% and 17.9% resistance rates were recorded against nalidixic acid, tetracycline, amoxacilin-clavulanic acid, cephalothin and ciprofloxacin, respectively. Multidrug resistance was observed among amikacin, amoxycillin-clavulanic acid, cephalothin, ciprofloxacin, streptomycin and tetracycline antimicrobials drugs.
Conclusions
The isolation of E. coli O157:H7 in raw meat and the existence of antimicrobial resistant isolates highlight the potential threat to public health. Hence implementation of E. coli O157:H7 prevention and control strategies from farm production to consumption of meat and meat products are crucial.
Publisher
Springer Science and Business Media LLC
Subject
Health, Toxicology and Mutagenesis,Public Health, Environmental and Occupational Health,Food Science
Reference41 articles.
1. Abdalla MA, Siham E, Suliman YYH, Alian A: Microbial Contamination of sheep carcasses at El Kadero slaughter house Khartoum State, Sudan. J Vet Sci 2009, 48: 1–2.
2. Abong BO: Prevalence of Escherichia coli O157:H7 in water and meat and meat products and vegetables sold in the Eastern Cape Province of South Africa and its impact on the diarrheic conditions of HIV/AIDS patients. Dissertation. The University of Fort Hare. Cape Town, South Africa; 2008.
3. Acha PN, Szyfres B: Colibacilosis. In Zoonoses and Communicable Diseases Common to Man and Animals. 3rd edition. pp 90, Washington, D.C; 2001:106.
4. Ahemed A, Li J, Shiloach Y, Robbins JB, Szu SC: Safety and immunogenicity of Escherichia coli O157 O-specific polysaccharide conjugate vaccine in 2–5 year old children. J Infect Dis 2006,193(4):515–521. 10.1086/499821
5. Bacon RT, Belk KE, Sofos JN, Clayton RP, Reagan JO, Smith GC: Microbial populations on animal hides and beef carcasses at different stages of slaughter in plants employing multiple sequential interventions for decontamination. J Food Prot 2000,63(8):1080–1086.
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