Author:
Yehia Hany M,AL-Dagal Mosffer M
Abstract
Abstract
Background
Campylobacter is a foodborne pathogen that is commonly associated with chicken. The aim of this work was to evaluate the prevalence of Campylobacter jejuni (as affected by refrigerated storage) in chicken samples obtained from the wholesale poultry market in the northern part of Riyadh City, Saudi Arabia.
Findings
A gradual increase in the number of positive samples was noted during storage at 4°C. On days 1, 3, and 7, the number of positive samples were 10 (30.305%), 15 (45.45%), and 27 (81.81%), respectively. Of 99 tested samples, 52 (52.25%) were positive for Campylobacter jejuni. Protein profiling by Sodium dodecyl sulfate -Polyacrylamide gel electrophoresis (SDS-PAGE) was used to identify Campylobacter jejuni. The results were verified using Analytical Profile Index (API Campy system, Marcy l’Etoile, France). Forty-three (82.69%) positive isolates were identified as C. jejuni subsp. jejuni 2, 5 isolates as C. jejuni subsp. jejuni 1 (9.61%), and 4 isolates as C. jejuni subsp. doylei (7.69).
Conclusion
C. jejuni positive samples increased rapidly during storage at 4°C for approximately 1 wk. Our results also indicated a connection between the protein profiles on SDS-PAGE and API Campy used for the identification of C. jejuni.
Publisher
Springer Science and Business Media LLC
Subject
Health, Toxicology and Mutagenesis,Public Health, Environmental and Occupational Health,Food Science
Cited by
11 articles.
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