A novel PCR-based point-of-care method enables rapid, sensitive and reliable diagnosis of Babesia gibsoni infection in dogs

Author:

Liu I-Li,Chi Nai-Yu,Chang Chia-Ling,Hung Ming-Long,Chiu Chun-Ta,Chen Hui-WenORCID

Abstract

Abstract Background Babesia gibsoni (B. gibsoni) is an intraerythrocytic protozoan parasite of dogs that causes fever and hemolytic illness. A timely diagnosis is essential for the disease management. Results Here, we report a QubeMDx PCR system which enables a rapid, sensitive and reliable diagnosis of B. gibsoni near the dog patient. Within 30 min, this diagnostic assay was able to detect as low as 0.002% parasitemia of the dog blood. Using clinical samples, this new assay was validated to demonstrate 100% agreement with real-time PCR. Conclusions This novel diagnostic method provides a reliable point-of-care test to assist in the identification of B. gibsoni.

Funder

Credo Biomedical Pte. Ltd.

National Taiwan University

Publisher

Springer Science and Business Media LLC

Subject

General Veterinary,General Medicine

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