Abstract
Abstract
Background
In human and murine obesity, adipose tissue dwelling macrophages and adipocytes produce monocyte chemoattractant protein-1 (MCP-1) leading to systemic low-grade inflammation. The aim of the study was to validate a canine MCP-1 ELISA assay for use in cats and to investigate whether a difference in MCP-1 concentrations could be detected between: a) cats having normal or elevated circulating serum amyloid A (SAA) levels and b) normal weight and obese cats. Serum obtained from 36 client-owned cats of various breed, age and sex with normal (n = 20) to elevated SAA (n = 16) was used for the validation of the canine MCP-1 ELISA assay. As no golden standard exists for measurement of inflammation, circulating MCP-1 concentrations were compared to SAA measurements, as an indicator of systemic inflammation. Analytical precision, dilution recovery and detection limit were calculated. A possible correlation between MCP-1 concentrations and obesity related measures (body fat percentage (BF%), insulin sensitivity and cytokine expression) were investigated in another population of 73 healthy, lean to obese, neutered domestic short-haired cats.
Results
Intra- (2.7–4.1%) and inter-assay (2.2–3.6%) coefficient of variation and dilution recovery were acceptable, and the detection limit was 27.1 pg/mL. MCP-1 did not correlate with SAA, and there was no difference between the inflammatory (SAA > 20 mg/L) and non-inflammatory group, due to a marked overlap in MCP-1 concentrations. Circulating MCP-1 concentrations were unaffected by BF% (r2 = 2.7 × 10–6, P = 0.21) and other obesity-related markers.
Conclusions
The present canine ELISA assay seems to be able to measure circulating feline MCP-1. However, further studies are needed to determine its possible use for detecting inflammation in relation to disease processes or obesity-related low-grade inflammation in cats.
Publisher
Springer Science and Business Media LLC
Subject
General Veterinary,General Medicine
Reference77 articles.
1. German AJ. The growing problem of obesity in dogs and cats. J Nutr. 2006;136:1940S-1946S.
2. Lund EM, Armstrong PJ, Kirk CA, Klausner JS. Prevalence and risk factors for obesity in adult cats from private US veterinary practices. Int J Appl Res Vet Med. 2006;3:88–96.
3. Banfield Applied Research and Knowledge. Overweight pets. State Pet Health 2017 Rep Data 2017. https://www.banfield.com/Banfield/media/PDF/Downloads/soph/2017-SOPH-Infographic.pdf. Accessed 17 December, 2021.
4. Öhlund M, Palmgren M, Holst BS. Overweight in adult cats: a cross-sectional study. Acta Vet Scand. 2018;60:1–10. https://doi.org/10.1186/s13028-018-0359-7.
5. Strissel KJ, Stancheva Z, Miyoshi H, Perfield JW, DeFuria J, Jick Z, et al. Adipocyte death, adipose tissue remodeling, and obesity complications. Diabetes. 2007;56:2910–8. https://doi.org/10.2337/db07-0767.