Combined with UPLC-Triple-TOF/MS-based plasma lipidomics and molecular pharmacology reveals the mechanisms of schisandrin against Alzheimer’s disease
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Published:2023-02-06
Issue:1
Volume:18
Page:
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ISSN:1749-8546
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Container-title:Chinese Medicine
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language:en
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Short-container-title:Chin Med
Author:
Zhao Tian-tian, Zhang Ying, Zhang Cheng-qin, Chang Ya-fei, Cui Mei-rong, Sun Yue, Hao Wen-qian, Yan Yu-meng, Gu Shuo, Xie Yao, Wei Bin-binORCID
Abstract
Abstract
Background
Alzheimer’s disease (AD), a type of neurodegeneration disease, is characterized by Aβ deposition and tangles of nerve fibers. Schisandrin is one of the main components of Fructus Schisandrae Chinensis. Researches showed that schisandrin can improve the cognitive impairment and memory of AD mice, but the specific mechanism has not been fully elucidated.
Purpose
The purpose of this study is to investigate the possible mechanism of schisandrin in improving AD pathology.
Methods
The Morris water maze test was executed to detect spatial learning and memory. Ultra performance liquid chromatography-Triple time of flight mass spectrometry (UPLC-Triple-TOF/MS)-based plasma lipidomics was used to study the changes of plasma lipids. Moreover, we measured the levels of protein and mRNA expression of APOE and ABCA1 in the rat brains and in BV2 microglia.
Results
Our study found that schisandrin could improve learning and memory, and reduce Aβ deposition in AD rats. Furthermore, we found that schisandrin can improve plasma lipid metabolism disorders. Therefore, we hypothesized schisandrin might act via LXR and the docking results showed that schisandrin interacts with LXRβ. Further, we found schisandrin increased the protein and mRNA expression of LXR target genes APOE and ABCA1 in the brain of AD rats and in BV2 microglia.
Conclusion
Our study reveals the neuroprotective effect and mechanism of schisandrin improves AD pathology by activating LXR to produce APOE and ABCA1.
Funder
National Natural Science Foundation of China Science and Technology Program of Shenyang, China Natural Science Research Program Of Yichang city
Publisher
Springer Science and Business Media LLC
Subject
Complementary and alternative medicine,Pharmacology
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