Abstract
AbstractAt the present, the mechanism of chlorophyll degradation in response to ionizing irradiation in harvested fruits have not been examined. To understand the effect of electron beam (E-beam) irradiation on the chlorophyll degrading pathway in relation to chlorophyll degrading enzymes activity, reactive oxygen species (ROS) and antioxidant capacities of harvested mangoes stored at 13 °C for 16 days were studied. E-beam-treated fruit significantly suppressed the activities of chlorophyll degrading enzymes especially pheophytinase (PPH) and chlorophyll degrading peroxidase (Chl-POX) in the late stage of storage. This resulted in the chlorophyll content being maintained. However, E-beam irradiation did not affect the activities of chlorophyllase (Chlase) and magnesium de-chelatase (MD). The respiration rate, ethylene production, ROS accumulation (hydrogen peroxide [H2O2] and superoxide radical [O−.2]) immediately increased after E-beam treatment, following which they significantly decreased in comparison to the control. E-beam treatment enhanced the fruit’s antioxidant capacity by activating the activities of catalase (CAT) and ascorbate peroxidase (APX) and glutathione (GSH) content, and inactivated the activity of superoxide dismutase (SOD). Further, it did not affect the activity of glutathione reductase (GR) and glutathione disulfide (GSSG), vitamin C content, or total phenolic content. These results imply that E-beam treatment has the potential to delay chlorophyll degradation by suppressing the Chl-POX and PPH activities as well as reduce ROS production via CAT, APX, and SOD activities and GSH content.
Funder
Petch Pra Jom Klao Ph.D.’s Degree Research Scholarship from King Mongkut’s University of Technology Thonburi, Bangkok, Thailand
Publisher
Springer Science and Business Media LLC
Subject
Organic Chemistry,General Biochemistry, Genetics and Molecular Biology
Reference65 articles.
1. Ulloa JA, Guatemala GM, Arriola E, Escalona HB, Díaz L (2009) Estimation of the diffusivities of sodium chloride, potassium sorbate and sodium bisulphite in mango slices processed by hurdle technology. J Food Eng 91(2):211–216. https://doi.org/10.1016/j.jfoodeng.2008.08.032
2. Zheng M, Shi J, Shi J, Wang Q, Li Y (2013) Antimicrobial effects of volatiles produced by two antagonistic Bacillus strains on the anthracnose pathogen in postharvest mangos. Biol Control 65(2):200–206. https://doi.org/10.1016/j.biocontrol.2013.02.004
3. Kim Y, Lounds-Singleton AJ, Talcott ST (2009) Antioxidant phytochemical and quality changes associated with hot water immersion treatment of mangoes (Mangifera indica L.). Food Chem 115(3):989–993. https://doi.org/10.1016/j.foodchem.2009.01.019
4. Narayana CK, Pal RK, Roy, SK (1996) Effect of pre-storage treatments and temperature regimes on shelf-life and respiratory behaviour of ripe Baneshan mango. J Food Sci Technol 33(1):79–82. https://www.semanticscholar.org/paper/Effect-of-pre-storage-treatments-and-temperature-on-Narayana-Pal/72ac164aad6f37c6e78198dbe81fb8d238ed5cd. Accessed 19 Nov 2019
5. Mercadante AZ, Rodriguez-amaya DB (1998) Effects of ripening, cultivar differences, and processing on the carotenoid composition of mango. J Agric Food Chem 8561(97):128–130. https://doi.org/10.1021/jf9702860
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