Abstract
AbstractConsistent production of bioactives from microbial sources remains a big challenge for fermentation based bio-processes. Setomimycin, a rare 9,9'-bianthrylanthracene antibiotic reported to be active against Gram positive bacteria i.e. Staphyloccocus aureus, Bacillus subtilis, Bacillus cereus, and Mycobacterium smegmatis, including mycobacteria is one of the least exploited antibiotic. Present work aims to enhance and maximize setomimycin production using One Factor at a Time (OFAT) approach, followed by Taguchi L9 orthogonal array (OA) design in 30L fermenter. Four most influential parameters, namely carbon source, nitrogen source, air and agitation were selected for optimization studies. The optimized production medium supplemented with 150 g/L glycerol and 7.5 g/L soyabean meal with an agitation rate of 100 RPM and air flow rate of 20 LPM (Liters Per Minute) resulted in 675 mg/L setomimycin production within 96–108 h of fermentation as compared to the initial production i.e. 40 mg/L. Thus, an overall enhancement of 16.8 folds was achieved in setomimycin production after optimization in 30L fermenter.
Graphical Abstract
Publisher
Springer Science and Business Media LLC
Subject
Applied Microbiology and Biotechnology,Biophysics
Reference29 articles.
1. Azmi AS, Ngoh CG, Mel M (2011) Prediction of significant factors in the production of ethanol by Ragi Tapai co-culture using Taguchi methodology. Afr J Biotechnol 10(81):18833–18841
2. Bhattacharya A, Das S, Majumder P, Batish A (2009) Estimating the effect of cutting parameters on surface finish and power consumption during high speed machining of AISI 1045 steel using Taguchi design and ANOVA. Prod Eng 3(1):31–40
3. Dong Y, Ding W, Sun C, Ji X, Ling C, Zhou Z, Chen Z, Chen X, Ju J (2020) Julichrome Monomers from marine gastropod mollusk-associated Streptomyces and Stereochemical Revision of Julichromes Q3⋅ 5 and Q3⋅ 3. Chem Biodivers 4:e2000057. https://doi.org/10.1002/cbdv.202000057
4. El-Housseiny GS, Ibrahim AA, Yassien MA, Aboshanab KM (2021) Production and statistical optimization of Paromomycin by Streptomyces rimosus NRRL 2455 in solid state fermentation. BMC Microbiol 1:1–3. https://doi.org/10.1186/s12866-021-02093-6
5. Elisashvili V, Parlar H, Kachlishvili E, Chichua D, Bakradze M, Kokhreidze N (2001) Ligninolytic activity of basidiomycetes grown under submerged and solid-state fermentation on plant raw material (sawdust of grapevine cuttings). Adv Food Sci 23(3):117–123