Generation of a recombinant chickenized monoclonal antibody against the neuraminidase of H9N2 avian influenza virus-Reference-Cited by-同舟云学术

Generation of a recombinant chickenized monoclonal antibody against the neuraminidase of H9N2 avian influenza virus

Published:2020-08-20 Issue:1 Volume:10 Page:
ISSN:2191-0855
Container-title:AMB Express
language:en
Short-container-title:AMB Expr

Author:

Wang Fei,Wang Yajuan,Wan Zhimin,Shao Hongxia,Qian Kun,Ye Jianqiang,Qin Aijian^ORCID

Abstract

AbstractWe previously reported a monoclonal antibody (mAb), 1G8, against the neuraminidase (NA) of H9N2 avian influenza virus (AIV) with significant NA inhibitory activity. To generate a recombinant chickenized mAb (RCmAb) against the NA of H9N2 AIV for passive immunization in poultry, the gene of the fragment of antigen binding (Fab) of mAb 1G8 was cloned and fused with the fragment crystallizable (Fc) gene of chicken IgY. The RCmAb 1G8 was expressed in COS-1 cells and could be detected in cell culture supernatant. The results of NA inhibitory activity tests of the RCmAb 1G8 in an enzyme-linked lectin assay (ELLA) and a microneutralization (MN) assay showed that the RCmAb 1G8 maintained significant NA inhibitory activity and neutralizing ability. This is the first chickenized antibody against AIV, which would be a good candidate for passive immunization in poultry.

Funder

Key Technologies Research and Development Program

Publisher

Springer Science and Business Media LLC

Subject

Applied Microbiology and Biotechnology,Biophysics

Link

https://link.springer.com/content/pdf/10.1186/s13568-020-01086-4.pdf

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