Author:
Moreno-Indias Isabel,Dodds Alister W,Argüello Anastasio,Castro Noemi,Sim Robert B
Abstract
Abstract
Background
The aim of the present study was to develop a haemolytic assay for the study of the complement system in dairy goats (Capra aegagrus hircus) and to characterize the major goat complement system proteins.
Results
The commonly used sheep erythrocyte sensitized with rabbit antibodies were not sensitive to lysis by goat serum, but the combination of human red blood cells (RBC) plus rabbit antibodies was the best option found for goat complement assay. A buffer based on HEPES instead of the classical veronal (barbitone) was developed. Three proteins were isolated: factor H, C1q and C3 and these were compared with the corresponding human proteins. A novel affinity chromatography technique was developed for isolation of factor H.
Conclusions
Human RBC plus rabbit antibodies were a suitable option for haemolytic assays. The isolated proteins are similar to the human counterparts.
Publisher
Springer Science and Business Media LLC
Subject
General Veterinary,General Medicine
Cited by
19 articles.
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