A Bayesian method for calculating real-time quantitative PCR calibration curves using absolute plasmid DNA standards

Author:

Sivaganesan Mano,Seifring Shawn,Varma Manju,Haugland Richard A,Shanks Orin C

Publisher

Springer Science and Business Media LLC

Subject

Applied Mathematics,Computer Science Applications,Molecular Biology,Biochemistry,Structural Biology

Reference35 articles.

1. Sambrook J, Russell DW: Molecular Cloning: A Laboratory Manual. Volume 1–3. 4th edition. New York: Cold Spring Harbor Laboratory Press; 2001.

2. Higuchi R, Fockler C, Dollinger G, Watson R: Kinetic PCR analysis: real-time monitoring of DNA amplification reactions. Biotechnology 1993, 11: 1026–1030. 10.1038/nbt0993-1026

3. ABI: Essentials of Real Time PCR. Applied Biosystems 2006.

4. Fierer N, Jackson JA, Vilgalys R, Jackson RB: Assessment of Soil Microbial Community Structure by Use of Taxon-Specific Quantitative PCR Assays. Applied and Environmental Microbiology 2005, 71(7):4117–4120. 10.1128/AEM.71.7.4117-4120.2005

5. ABI: Absolute Quantitation Using Standard Curve Getting Started Guide. Applied Biosystems 2006.

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