Assessment of Soil Microbial Community Structure by Use of Taxon-Specific Quantitative PCR Assays-Reference-Cited by-同舟云学术

Assessment of Soil Microbial Community Structure by Use of Taxon-Specific Quantitative PCR Assays

Published:2005-07 Issue:7 Volume:71 Page:4117-4120
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Fierer Noah¹,Jackson Jason A.¹,Vilgalys Rytas¹,Jackson Robert B.¹²

Affiliation:

1. Department of Biology, Duke University, Durham, North Carolina 27708

2. Nicholas School of the Environment and Earth Sciences, Duke University, Durham, North Carolina 27708

Abstract

ABSTRACT Here we describe a quantitative PCR-based approach to estimating the relative abundances of major taxonomic groups of bacteria and fungi in soil. Primers were thoroughly tested for specificity, and the method was applied to three distinct soils. The technique provides a rapid and robust index of microbial community structure.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.71.7.4117-4120.2005

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