Author:
Xu Guangxin,Ying Feng,Wu Huangming,Tang Xixiang
Abstract
Abstract
Background
Gray blight is among the most destructive diseases that affect tea plants worldwide. In this study, microorganisms from deep-sea sediment samples for those with antagonistic effects were screened against gray blight caused by Pestalotiopsis theae.
Results
Thirty-two and twenty-eight morphologically different deep-sea bacteria and fungi were isolated, respectively. Isolates B5 and A65 clearly inhibited the pathogens in vitro and were prepared as wettable agent powders for evaluation in micro-plot field trials. Foliar application of the 48-h culture of B5 (1 × 108, 2 × 107, 1 × 107 colony-forming units (CFU)/ml) significantly reduced the incidence of gray blight disease. Compared to the untreated control, spraying with B5 inhibited gray blight disease by 78.57%. Isolate B5 was identified as Bacillus subtilis B5 in morphological and 16S rDNA sequence analyses. The foliar application of 7-day cultures of A65 (1 × 108, 2 × 107, 1 × 107 CFU/ml) significantly reduced the incidence of gray blight disease. A65 (108 CFU/ml) inhibited gray blight disease by 75.46% and was identified as Paecilomyces lilacinus A65 in morphologically and internally transcribed spacer sequence analyses.
Conclusions
These candidate microbial pesticides may inhibit gray wilt in tea, replace chemical pesticides’ use without causing environmental pollution, and promote the development of green agriculture.
Funder
Tobacco Technology Center Project of Fujian Province
Henan Provincial Key Laboratory of Materials on Deep‐Earth Engineering
Asian Countries Maritime Cooperation Fund
Special Support Project for Southern Marine Science and Engineering Guangdong Laboratory
Publisher
Springer Science and Business Media LLC
Subject
Insect Science,Plant Science,Agronomy and Crop Science,Ecology
Cited by
1 articles.
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