Author:
Krishnaswami Venkataraman,Van Noorden Cornelis J F,Manders Erik M M,Hoebe Ron A
Abstract
Abstract
Background
Optical nanoscopy based on separation of single molecules by stochastic switching and subsequent localization allows surpassing the diffraction limit of light. The growing pursuit towards live-cell imaging using nanoscopy demands advancements in both science and technology.
Results
In this article, we provide an overview of the technological advancements in the development of scientific cameras used for nanoscopy. We discuss the prospects of novel digital photon counting cameras based on a single-photon avalanche diode (SPAD) array camera for optical nanoscopy. Numerical simulations are used to evaluate and compare different scientific cameras for their performance towards single-molecule identification and localization.
Conclusion
A SPAD array camera with single-photon sensitivity and zero read-out noise allows for the detection of extremely weak signals at ultra-fast imaging speeds. With temporal resolution in the order of micro-seconds, a SPAD array camera offers great potential for live-cell imaging with super-resolution.
Publisher
Springer Science and Business Media LLC
Subject
Atomic and Molecular Physics, and Optics
Reference47 articles.
1. Barsic A, Piestun R: Super-resolution of dense nanoscale emitters beyond the diffraction limit using spatial and temporal information. Appl Phys Lett 2013, 102: 231103. 10.1063/1.4809834
2. Basden AG, Haniff CA, Mackay CD: Photon counting strategies with low-light-level CCDs. Mon Notices R Astron Soc 2003, 345: 985–991. 10.1046/j.1365-8711.2003.07020.x
3. Betzig E, Patterson GH, Sougrat R, Lindwasser OW, Olenych S, Bonifacino JS, Davidson MW, Lippincott-Schwartz J, Hess HF: Imaging intracellular fluorescent proteins at nanometer resolution. Science 2006, 313: 1642–1645. 10.1126/science.1127344
4. Blanc N, Giffard P, Seitz P, Buchschacher P, Nguyen V, Hoheisel M: Semiconductor Image Sensing. In More than Moore: creating high value micro/nanoelectronics systems. 1st edition. Edited by: Zhang QG, Roosmalen A. US: Springer; 2009:239–278.
5. Burri S, Stucki D, Maruyama Y, Bruschini C, Charbon E, Regazzoni F: Jailbreak imagers: transforming a single-photon image sensor into a true random number generator. Snowbird Resort, Utah, USA: Paper presented at 14th International Image Sensor Workshop; 2013:12–16.
Cited by
25 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献