Author:
Barrit Thibault,Campion Claire,Aligon Sophie,Bourbeillon Julie,Rousseau David,Planchet Elisabeth,Teulat Béatrice
Abstract
Abstract
Background
Seedling growth is an early phase of plant development highly susceptible to environmental factors such as soil nitrogen (N) availability or presence of seed-borne pathogens. Whereas N plays a central role in plant-pathogen interactions, its role has never been studied during this early phase for the interaction between Arabidopsis thaliana and Alternaria brassicicola, a seed-transmitted necrotrophic fungus. The aim of the present work was to develop an in vitro monitoring system allowing to study the impact of the fungus on A. thaliana seedling growth, while modulating N nutrition.
Results
The developed system consists of square plates placed vertically and filled with nutrient agar medium allowing modulation of N conditions. Seeds are inoculated after sowing by depositing a droplet of conidial suspension. A specific semi-automated image analysis pipeline based on the Ilastik software was developed to quantify the impact of the fungus on seedling aerial development, calculating an index accounting for every aspect of fungal impact, namely seedling death, necrosis and developmental delay. The system also permits to monitor root elongation. The interest of the system was then confirmed by characterising how N media composition [0.1 and 5 mM of nitrate (NO3−), 5 mM of ammonium (NH4+)] affects the impact of the fungus on three A. thaliana ecotypes. Seedling development was strongly and negatively affected by the fungus. However, seedlings grown with 5 mM NO3− were less susceptible than those grown with NH4+ or 0.1 mM NO3−, which differed from what was observed with adult plants (rosette stage).
Conclusions
The developed monitoring system allows accurate determination of seedling growth characteristics (both on aerial and root parts) and symptoms. Altogether, this system could be used to study the impact of plant nutrition on susceptibility of various genotypes to fungi at the seedling stage.
Funder
French regional council program ‘Objectif Végétal, Research, Education and Innovation in Pays de la Loire’ supported by the French Region Pays de la Loire, Angers Loire Métropole
Ministère de l'Agriculture et de l'Alimentation
Conseil Régional des Pays de la Loire
Agence Nationale de la Recherche, programme Investissements d'avenir, project PHENOME
Publisher
Springer Science and Business Media LLC
Subject
Plant Science,Genetics,Biotechnology
Cited by
1 articles.
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