Nitrogen Nutrition Modulates the Response to Alternaria brassicicola Infection via Metabolic Modifications in Arabidopsis Seedlings

Author:

Barrit Thibault1,Planchet Elisabeth1,Lothier Jérémy1,Satour Pascale1,Aligon Sophie1,Tcherkez Guillaume12ORCID,Limami Anis M.1ORCID,Campion Claire1ORCID,Teulat Béatrice1ORCID

Affiliation:

1. Institut Agro, University of Angers, INRAE, IRHS, SFR QUASAV, 49000 Angers, France

2. Research School of Biology, Australian National University, Canberra, ACT 2601, Australia

Abstract

Little is known about the effect of nitrogen nutrition on seedling susceptibility to seed-borne pathogens. We have previously shown that seedlings grown under high nitrate (5 mM) conditions are less susceptible than those grown under low nitrate (0.1 mM) and ammonium (5 mM) in the Arabidopsis-Alternaria brassicicola pathosystem. However, it is not known how seedling metabolism is modulated by nitrogen nutrition, nor what is its response to pathogen infection. Here, we addressed this question using the same pathosystem and nutritive conditions, examining germination kinetics, seedling development, but also shoot ion contents, metabolome, and selected gene expression. Nitrogen nutrition clearly altered the seedling metabolome. A similar metabolomic profile was observed in inoculated seedlings grown at high nitrate levels and in not inoculated-seedlings. High nitrate levels also led to specific gene expression patterns (e.g., polyamine metabolism), while other genes responded to inoculation regardless of nitrogen supply conditions. Furthermore, the metabolites best correlated with high disease symptoms were coumarate, tyrosine, hemicellulose sugars, and polyamines, and those associated with low symptoms were organic acids (tricarboxylic acid pathway, glycerate, shikimate), sugars derivatives and β-alanine. Overall, our results suggest that the beneficial effect of high nitrate nutrition on seedling susceptibility is likely due to nutritive and signaling mechanisms affecting developmental plant processes detrimental to the pathogen. In particular, it may be due to a constitutively high tryptophan metabolism, as well as down regulation of oxidative stress caused by polyamine catabolism.

Funder

French Region Pays de la Loire, Angers Loire Métropole

National Agronomy School “L’Institut Agro Rennes-Angers”

French Ministry of Agriculture and Food and the regional Council of Pays de la Loire

Publisher

MDPI AG

Reference63 articles.

1. Nonlinear response of N2O flux to incremental fertilizer addition in a continuous maize (Zea mays L.) cropping system;McSwiney;Glob. Chang. Biol.,2005

2. Human alteration of the global nitrogen cycle: Sources and consequences;Vitousek;Ecol. Appl.,1997

3. Plant nutrient management for enhanced productivity in intensive grain production systems of the United States and Asia;Dobermann;Plant Soil,2002

4. Coastal nitrogen pollution: A review of sources and trends globally and regionally;Howarth;Harmful Algae,2008

5. Terrestrial pesticide exposure of amphibians: An underestimated cause of global decline?;Schmidt;Sci. Rep.,2013

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3