i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases-Reference-Cited by-同舟云学术

i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases

Published:2018-02-26 Issue:1 Volume:19 Page:
ISSN:1474-760X
Container-title:Genome Biology
language:en
Short-container-title:Genome Biol

Author:

Ohtsuka Masato^ORCID,Sato Masahiro,Miura Hiromi,Takabayashi Shuji,Matsuyama Makoto,Koyano Takayuki,Arifin Naomi,Nakamura Shingo,Wada Kenta,Gurumurthy Channabasavaiah B.

Funder

Japan Society for the Promotion of Science

Tokai University School of Medicine Research Aid

Tokai University School of Medicine Project Research

Publisher

Springer Science and Business Media LLC

Link

http://link.springer.com/content/pdf/10.1186/s13059-018-1400-x.pdf

Reference31 articles.

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2. Wang H, Yang H, Shivalila CS, Dawlaty MM, Cheng AW, Zhang F, et al. One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell. 2013;153:910–8.

3. Gurumurthy CB, Grati M, Ohtsuka M, Schilit SLP, Quadros RM, Liu XZ. CRISPR: a versatile tool for both forward and reverse genetics research. Hum Genet. 2016;135:971–6.

4. Hashimoto M, Yamashita Y, Takemoto T. Electroporation of Cas9 protein/sgRNA into early pronuclear zygotes generates non-mosaic mutants in the mouse. Dev Biol. 2016;418:1–9.

5. Kaneko T, Mashimo T. Simple genome editing of rodent intact embryos by electroporation. PLoS One. 2015;10:e0142755.

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