Author:
Joo JiHoon E,Wong Ee Ming,Baglietto Laura,Jung Chol-Hee,Tsimiklis Helen,Park Daniel J,Wong Nicholas C,English Dallas R,Hopper John L,Severi Gianluca,Giles Graham G,Southey Melissa C
Abstract
Abstract
Background
Dried blood (Guthrie card) spots provide an efficient way to collect and store blood specimens. DNA from this source has been utilised for a number of molecular analyses including genome-wide association studies, but only few studies have tested the feasibility of using it for epigenetic applications, particularly at a genome-wide level.
Results
In this study, we demonstrate the successful use of DNA isolated from archived dried blood spots for the Infinium HumanMethylation450 Beadchip, along with DNA from matched frozen buffy coats. We obtained high quality and reproducible genome-wide DNA methylation profiles using both sample types. We also report high correlations (r > 0.9907) between DNA obtained from matched dried blood spots and frozen buffy coats, sufficient to distinguish between unrelated individuals.
Conclusions
We, thus, demonstrate that DNA from archived dried blood spots is suitable for genome-wide DNA methylation profiling.
Publisher
Springer Science and Business Media LLC
Cited by
57 articles.
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