Abstract
AbstractFungi of the genus Trichoderma are routinely used as biocontrol agents and for the production of industrial enzymes. Trichoderma spp. are interesting hosts for heterologous gene expression because their saprotrophic and mycoparasitic lifestyles enable them to thrive on a large number of nutrient sources and some members of this genus are generally recognized as safe (GRAS status). In this review, we summarize and discuss several aspects involved in heterologous gene expression in Trichoderma, including transformation methods, genome editing strategies, native and synthetic expression systems and implications of protein secretion. This review focuses on the industrial workhorse Trichoderma reesei because this fungus is the best-studied member of this genus for protein expression and secretion. However, the discussed strategies and tools can be expected to be transferable to other Trichoderma species.
Funder
Österreichische Forschungsförderungsgesellschaft
Technische Universität Wien
Publisher
Springer Science and Business Media LLC
Subject
Cell Biology,Molecular Biology,Applied Microbiology and Biotechnology,Ecology, Evolution, Behavior and Systematics,Biotechnology
Cited by
17 articles.
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