Schnelles und effizientes Genom-Editieren in Aureobasidium

Abstract

AbstractThe fungus Aureobasidium pullulans is used in the industry to produce pullulan and polymalate and possesses some outstanding properties such as polyextremotolerance and an unusual life cycle. This makes A. pullulans an interesting study object, for which we need an efficient genome editing method. This article describes how CRISPR/Cas can be used for this purpose. In particular, the usage of in vitro assembled ribonucleoproteins proved to be a fast and efficient method for genome editing.