Fragmentation of DNA affects the accuracy of the DNA quantitation by the commonly used methods

Author:

Sedlackova Tatiana,Repiska Gabriela,Celec Peter,Szemes Tomas,Minarik Gabriel

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology

Reference23 articles.

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2. Wyllie AH: Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature. 1980, 284: 555-556. 10.1038/284555a0.

3. Jahr S, Hentze H, Englisch S, Hardt D, Fackelmayer FO, Hesch RD, Knippers R: DNA fragments in the blood plasma of cancer patients: quantitations and evidence for their origin from apoptotic and necrotic cells. Cancer Res. 2001, 61: 1659-1665.

4. Kanduc D, Mittelman A, Serpico R, Sinigaglia E, Sinha AA, Natale C, Santacroce R, Di Corcia MG, Lucchese A, Dini L, Pani P, Santacroce S, Simone S, Bucci R, Farber E: Cell death: apoptosis versus necrosis (review). Int J Oncol. 2002, 21: 165-170.

5. Shokere LA, Holden MJ, Jenkins GR: Comparison of fluorometric and spectrophotometric DNA quantification for real-time quantitative PCR of degraded DNA. Food Control. 2009, 20: 391-401. 10.1016/j.foodcont.2008.07.009.

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