Author:
Noh Gyeong Eon,Rho Sum,Chang Yong Jin,Min Byung Hwa,Kim Jong-Myoung
Abstract
Abstract
Background
Prolactin (PRL) is a key hormone for osmoregulation in fish. Levels of PRL in the pituitary gland and plasma ion composition of clownfish seem to change to regulate their hydromineral balance during adaptation to waters of different salinities. In order to understand osmoregulatory mechanism and its association with growth performance and PRL in fish, the gene encoding PRL and its expression level in cinnamon clownfish Amphiprion melanopus upon acclimation to low salinity was analyzed.
Results
The PRL gene of A. melanopus encoded a protein of 212 amino acid residues comprised of a putative signal peptide of 24 amino acids and a mature protein of 188 amino acids. Analysis of growth performance under different salinities of 34, 25, 15, and 10 ppt indicated that cinnamon clownfish could survive under salinities as low as 10 ppt. A higher rate of growth was observed at the lower salinities as compared to that of 34 ppt. Upon shifting the salinity of the surrounding water from 34 ppt to 15 ppt, the level of the PRL transcripts gradually increased to reach the peak level until 24 h of acclimation at 15 ppt, but decreased back as adaptation continued to 144 h. In contrast, levels of plasma Na+, Cl-, and osmolality decreased at the initial stage (4–8 h) of acclimation at 15 pt but increased back as adaptation continued till 144 h.
Conclusion
Cinnamon clownfish could survive under salinities as low as 10 ppt. Upon shifting the salinity of the surrounding water from 34 ppt to 15 ppt, the level of the PRL transcripts gradually increased during the initial stage of acclimation but decreased back to the normal level as adaptation continued. An opposite pattern of changes - decrease at the beginning followed by an increase - in the levels of plasma Na+, Cl-, and osmolality was found upon acclimation to low salinity. The results suggest an involvement of PRL in the processes of osmoregulation and homeostasis in A. melanopus.
Publisher
Springer Science and Business Media LLC
Subject
Water Science and Technology,Aquatic Science,Ecology,Ecology, Evolution, Behavior and Systematics
Reference38 articles.
1. Moorhead JA, Zeng C: Development of captive breeding techniques for marine ornamental fish: a review. Rev Fish Sci. 2000, 18: 315-343.
2. Wabnitz C, Taylor M, Green E, Razak T: From Ocean to aquarium: The global trade in marine ornamental species. 2003, Cambridge, UK: UNEP-WCMC
3. Bœuf G, Payan P: How should salinity influence fish growth?. Comp Biochem Physiol C. 2001, 130: 411-423. 10.1016/S1095-6433(01)00439-1.
4. Specker JL, Schreiber AM, McArdle ME, Poholek A, Henderson J, Bengtson DA: Metamorphosis in summer flounder: effects of acclimation to low and high salinities. Aquaculture. 1999, 176: 145-154. 10.1016/S0044-8486(99)00057-5.
5. Hoff FH: Conditioning, spawning and rearing of fish with emphasis on marine clownfish. 1996, Dade City, FL: Aquaculture Consultants Inc
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