BIOLOGICAL EFFECTS OF OLIGOSACCHARIDE CHONDROITIN SULFATE C ON HUMAN ARTICULAR CHONDROCYTES

Author:

Chen Wei-Chuan1,Wei Yu-Hong2,Huang Jui-Bin1,Chu I-Ming1,Yao Chao-Ling3

Affiliation:

1. Department of Chemical Engineering, National Tsing Hua University, Hsinchu 300, Taiwan

2. Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Chung-Li, Taoyuan 320, Taiwan

3. Department of Chemical Engineering and Materials Science, Yuan Ze University, Chung-Li, Taoyuan 320, Taiwan

Abstract

Chondroitin sulfate C (CSC) is an important extracellular matrix (ECM) component of native cartilage tissue. Since ECM is considered to play an important role in guiding proper cellular functions, such as proliferation, differentiation, migration, synthesis or degradation of ECM, in specific tissues, we would like to elucidate the effects of CSC on chondrocytes are cultured on type-II collagen (COL II) scaffolds in this study. In particular, we want to investigate if the oligosaccharides of CSC (O-CSC) have much stronger effects on the chondrocytes. In this in vitro study, human articular chondrocytes were cultured on porous scaffolds made of COL II, cross-linked by genipin. Media containing different molecular weights of CSC were used to cultivate the cells. The results were examined mainly from the gene expression profiles of the cultured cells. The expression levels of several genes were examined by real-time PCR. These included genes of COL II, aggrecan, SRY-related high mobility group-box gene 9 (SOX9) and cartilage oligo matrix protein (COMP), alkaine phosphtase (ALP), a disintegrin and metalloproteinases with thrombospondin motifs 4 (ADAMTS-4), a disintegrin and metalloproteinases with thrombospondin motifs 5 (ADAMTS-5), matrix metalloproteinases 3 (MMP-3) and tissue inhibitors of metalloproteinases 3 (TIMP-3). The results suggested that O-CSC is more potent in upregulating genes that promote chondrogenesis and downregulating genes that degrade cartilage ECM. The results suggest low-molecular-weight glycosaminoglycans may have therapeutic values in osteoarthritis treatment and may lead to further understanding of the basic mechanism of the interactions between the chondrocytes and their ECM.

Publisher

National Taiwan University

Subject

Biomedical Engineering,Bioengineering,Biophysics

Cited by 3 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3