A long-term culture system based on a collagen vitrigel membrane chamber that supports liver-specific functions of hepatocytes isolated from mice with humanized livers

Author:

Watari Ryuji12,Kakiki Motoharu1,Oshikata Ayumi3,Takezawa Toshiaki3,Yamasaki Chihiro4,Ishida Yuji4,Tateno Chise4,Kuroda Yukie5,Ishida Seiichi5,Kusano Kazutomi1

Affiliation:

1. Drug Metabolism and Pharmacokinetics Tsukuba, Global Drug Metabolism and Pharmacokinetics, Biopharmaceutical Assessments Core Function Unit, Medicine Development Center, Eisai Co., Ltd.

2. Department of Genomics-Based Drug Discovery, Graduate School of Comprehensive Human Sciences, University of Tsukuba

3. Division of Biotechnology, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization

4. R&D Department, PhoenixBio Co., Ltd.

5. Division of Pharmacology, National Institute of Health Sciences

Publisher

Japanese Society of Toxicology

Subject

Toxicology

Reference21 articles.

1. Araki, T., Iwazaki, N., Ishiguro, N., Sakamoto, A., Nagata, K., Ohbuchi, M., Moriguchi, H., Motoi, M., Shinkyo, R., Homma, T., Sakamoto, S., Iwase, Y., Ise, R., Nakanishi, Y., Uto, M. and Inoue, T. (2016): Requirements for human iPS cell-derived hepatocytes as an alternative to primary human hepatocytes for assessing absorption, distribution, metabolism, excretion and toxicity of pharmaceuticals. Fundam. Toxicol. Sci., 3, 89-99.

2. Bell, C.C., Hendriks, D.F., Moro, S.M., Ellis, E., Walsh, J., Renblom, A., Fredriksson Puigvert, L., Dankers, A.C., Jacobs, F., Snoeys, J., Sison-Young, R.L., Jenkins, R.E., Nordling, Å., Mkrtchian, S., Park, B.K., Kitteringham, N.R., Goldring, C.E., Lauschke, V.M. and Ingelman-Sundberg, M. (2016): Characterization of primary human hepatocyte spheroids as a model system for drug-induced liver injury, liver function and disease. Sci. Rep., 6, 25187.

3. Boess, F., Kamber, M., Romer, S., Gasser, R., Muller, D., Albertini, S. and Suter, L. (2003): Gene expression in two hepatic cell lines, cultured primary hepatocytes, and liver slices compared to the in vivo liver gene expression in rats: possible implications for toxicogenomics use of in vitro systems. Toxicol. Sci., 73, 386-402.

4. den Braver-Sewradj, S.P., den Braver, M.W., Vermeulen, N.P., Commandeur, J.N., Richert, L. and Vos, J.C. (2016): Inter-donor variability of phase I/phase II metabolism of three reference drugs in cryopreserved primary human hepatocytes in suspension and monolayer. Toxicol. In Vitro, 33, 71-79.

5. Gebhardt, R., Hengstler, J.G., Müller, D., Glöckner, R., Buenning, P., Laube, B., Schmelzer, E., Ullrich, M., Utesch, D., Hewitt, N., Ringel, M., Hilz, B.R., Bader, A., Langsch, A., Koose, T., Burger, H.J., Maas, J. and Oesch, F. (2003): New hepatocyte in vitro systems for drug metabolism: metabolic capacity and recommendations for application in basic research and drug development, standard operation procedures. Drug Metab. Rev., 35, 145-213.

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