Enzymatic Method of Bioactive Peptides Production from Milk Protein Raw Materials: A Scoping Review

Abstract

Introduction: For 14 years, interest in the use of dairy raw materials for the production of bioactive peptides (BPs) with antihypertensive, antioxidant and antidiabetic effects has been in a phase of rapid growth because of the need to prevent different diseases. Particular attention is being paid to enzymatic production method, as it allows for a targeted process of BP extraction with specified properties.The  purpose of this scoping review was to summarize and systematize the experience of international research over the last 14 years in the field of FG as a method to extract BPs from milk protein substrates.Materials and Methods: This scoping review was performed in accordance with the PRISMA-ScR guidelines. Scientific papers were searched in the Google Scholar database, because allows searching for scientific sources without restrictions on their format and type of scientific data presentation (book, dissertation, article), as well as includes scientific sources from other databases and performs relevance sorting.Results:  The search identified 72 sources published between 2007 and 2021 in English. Included sources are devoted to hydrolysis by starter bacteria enzymes (SBE) - 50%  and by commercial enzyme preparations (CEP) - 50%.Conclusion: There are a number of challenges that need to be addressed in order to increase the level of commercialization of BP production through directed hydrolysis. Creation of databases; building up the research base on co-fermentation by microorganisms and commercial enzymes; conducting PCR studies of microbial protease genes will increase the practical applicability of research and the validity of conducting more evidence-based in vivo and clinical studies.