Desmin cytoskeletal modifications after a bout of eccentric exercise in the rat

Abstract

Desmin content and immunohistochemical appearance were measured in tibialis anterior muscles of rats subjected to a single bout of 30 eccentric contractions (ECs). Ankle torque was measured before EC and at various recovery times, after which immunohistochemical and immunoblot analyses were performed. Torque decreased by ∼50% immediately after EC and fully recovered 168 h later ( P < 0.001). Loss of desmin staining was maximal 12 h after EC and recovered by 72 h. Immunoblots unexpectedly demonstrated a significant increase in the desmin-to-actin ratio by 72 h after EC ( P < 0.01) and was still increasing after 168 h ( P < 0.0001). These data demonstrate a relatively rapid qualitative loss of desmin immunostaining immediately after a single EC bout but a tremendous quantitative increase in desmin content 72–168 h later. This dynamic restructuring of the muscle's intermediate filament system may be involved in the mechanism of EC-induced muscle injury and may provide a structural explanation for the protective effects observed in muscle after a single EC bout.