Author:
Rivera Alicia,De Franceschi Lucia,Peters Luanne L.,Gascard Philippe,Mohandas Narla,Brugnara Carlo
Abstract
Moderate hemolytic anemia, abnormal erythrocyte morphology (spherocytosis), and decreased membrane stability are observed in mice with complete deficiency of all erythroid protein 4.1 protein isoforms (4.1−/−; Shi TS et al. J Clin Invest 103: 331, 1999). We have examined the effects of erythroid protein 4.1 (4.1R) deficiency on erythrocyte cation transport and volume regulation. 4.1−/−mice exhibited erythrocyte dehydration that was associated with reduced cellular K and increased Na content. Increased Na permeability was observed in these mice, mostly mediated by Na/H exchange with normal Na-K pump and Na-K-2Cl cotransport activities. The Na/H exchange of 4.1−/−erythrocytes was markedly activated by exposure to hypertonic conditions (18.2 ± 3.2 in 4.1−/−vs. 9.8 ± 1.3 mmol/1013cell × h in control mice), with an abnormal dependence on osmolality (EC50= 417 ± 42 in 4.1−/−vs. 460 ± 35 mosmol/kgH2O in control mice), suggestive of an upregulated functional state. While the affinity for internal protons was not altered (K0.5= 489.7 ± 0.7 vs. 537.0 ± 0.56 nM in control mice), the Vmaxof the H-induced Na/H exchange activity was markedly elevated in 4.1−/−erythrocytes ( Vmax91.47 ± 7.2 compared with 46.52 ± 5.4 mmol/1013cell × h in control mice). Na/H exchange activation by okadaic acid was absent in 4.1−/−erythrocytes. Altogether, these results suggest that erythroid protein 4.1 plays a major role in volume regulation and physiologically downregulates Na/H exchange in mouse erythrocytes. Upregulation of the Na/H exchange is an important contributor to the elevated cell Na content of 4.1−/−erythrocytes.
Publisher
American Physiological Society
Cited by
23 articles.
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