Author:
Yamamoto M.,Reeve J. R.,Keire D. A.,Green G. M.
Abstract
Pancreatic secretion of protein, water, chloride, and bicarbonate under basal conditions and in response to intravenous and intraduodenal stimuli were studied in awake rats fully recovered from surgery. During the basal phase of pancreatic secretion, protein output and water output were weakly correlated or uncorrelated, consistent with separate regulation and distinct cellular origin of enzyme (acinar cells) and water (duct cells), referred to as the two-component paradigm of pancreatic secretion. When pancreatic secretion was stimulated physiologically, water and protein output abruptly became strongly and significantly correlated, suggesting that protein secretion and water secretion are tightly coupled or that protein secretion is dependent on water secretion. The apparent function of this coupling is to resist or prevent increases in protein concentration as protein output increases. This pattern of secretion was reproduced by intravenous infusion of the CCK-58 form of cholecystokinin, which strongly stimulates pancreatic water and chloride secretion, but not by CCK-8, which only weakly stimulates water and chloride secretion in a non-dose-dependent manner. The remarkably tight association of water and protein secretion in food-stimulated and CCK-58-stimulated pancreatic secretion is consistent with a single cell type as the origin of both water and enzyme secretion, i.e., the acinar cell, and is not consistent with the two-component paradigm of pancreatic secretion. Because CCK-58 is the only detectable endocrine form of cholecystokinin in the rat and its bioactivity pattern is markedly and qualitatively different from CCK-8, actions previously recorded for CCK-8 should be reexamined.
Publisher
American Physiological Society
Subject
Physiology (medical),Gastroenterology,Hepatology,Physiology
Reference73 articles.
1. Alphin RSand Lin TM.Effect of feeding and sham feeding on pancreatic secretion of the rat.Am J Physiol197: 260–262, 1959.
2. Ultrastructural responses of rat exocrine pancreas to cholecystokinin octapeptide and secretin
3. Carew MA, Yang X, Schultz C, and Shears SB.myo-Inositol 3,4,5,6-tetrakisphosphate inhibits an apical calcium-activated chloride conductance in polarized monolayers of a cystic fibrosis cell line.J Biol Chem275: 26906–26913, 2000.
4. Case RMand Argent BE.Pancreatic secretion of electrolytes and water. In:Handbook of Physiology. The Gastrointestinal System. Salivary, Gastric, Pancreatic, and Hepatobiliary Secretion.Bethesda, MD: Am. Physiol. Soc. 1989, sect. 6, vol.III, chapt. 20, p. 383–417.
5. Case RMand Argent BE.Pancreatic duct cell secretion: control and mechanism of transport. In:The Pancreas: Biology, Pathology, and Disease, edited by Go VLW, DiMagno, EP, Gardner JD, Lebenthal E, Reber HA, and Scheele GA. New York: Raven, 1993, p. 301–350.
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