Activation of the Ae4 (Slc4a9) cation-driven Cl−/HCO3− exchanger by the cAMP-dependent protein kinase in salivary gland acinar cells-Reference-Cited by-同舟云学术

Activation of the Ae4 (Slc4a9) cation-driven Cl−/HCO3− exchanger by the cAMP-dependent protein kinase in salivary gland acinar cells

Published:2021-12-01 Issue:6 Volume:321 Page:G628-G638
ISSN:0193-1857
Container-title:American Journal of Physiology-Gastrointestinal and Liver Physiology
language:en
Short-container-title:American Journal of Physiology-Gastrointestinal and Liver Physiology

Author:

Peña-Münzenmayer Gaspar¹²,Kondo Yusuke³,Salinas Constanza⁴,Sarmiento José⁴,Brauchi Sebastián⁴²,Catalán Marcelo A.⁵⁴^ORCID

Affiliation:

1. Facultad de Ciencias, Instituto de Bioquímica y Microbiología, Universidad Austral de Chile, Valdivia, Chile

2. Millennium Nucleus of Ion Channel-Associated Diseases (MiNICAD), Valdivia, Chile

3. Division of Oral Reconstruction and Rehabilitation, Kyushu Dental University, Fukuoka, Japan

4. Facultad de Medicina, Instituto de Fisiología, Universidad Austral de Chile, Valdivia, Chile

5. Facultad de Ciencias de la Salud, Universidad Arturo Prat, Iquique, Chile

Abstract

We found that Ae4 exchanger activity in secretory salivary gland acinar cells is increased upon β-adrenergic receptor stimulation. The activation of Ae4 was prevented by H89, a nonselective PKA inhibitor. Protein sequence analysis revealed two residues (S173 and S273) that are potential targets of cAMP-dependent protein kinase (PKA). Experiments in CHO-K1 cells expressing S173A and S273A mutants showed that S173A, but not S273A, is not activated by PKA.

Funder

Government of Chile | National Agency of Investigation and Development (ANID) | Millennium Science Initiative

Fondequip

HHS | NIH | National Institute of Dental and Craniofacial Research

MINEDUC | CONICYT | Fondo Nacional de Desarrollo Científico y Tecnológico

Publisher

American Physiological Society

Subject

Physiology (medical),Gastroenterology,Hepatology,Physiology

Link

https://journals.physiology.org/doi/pdf/10.1152/ajpgi.00145.2021

Reference52 articles.

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