Subcutaneous abdominal preadipocyte differentiation in vitro inversely correlates with central obesity

Author:

Permana Paska A.,Nair Saraswathy,Lee Yong-Ho,Luczy-Bachman Georgia,Vozarova de Courten Barbora,Tataranni P. Antonio

Abstract

Expansion of adipose tissue mass results from increased number and size of adipocyte cells. We hypothesized that subcutaneous abdominal preadipocytes in obese individuals might have an intrinsically higher propensity to differentiate into adipocytes. Thus we investigated the relationship between obesity and the level of in vitro preadipocyte differentiation in Pima Indians. Subcutaneous abdominal stromal vascular fractions containing preadipocytes were cultured from 58 nondiabetic subjects [31 M/27 F, 30 ± 6 yr, body fat 34 ± 8% by dual-energy X-ray absorptiometry (means ± SD)]. The average percentage of preadipocyte differentiation (PDIFF; cell count by microscopy) was 11 ± 11% (range 0.2–51%). PDIFF correlated negatively with percent body fat ( r = −0.35, P = 0.006) and waist circumference ( r = −0.45, P = 0.0004). Multiple regression analysis indicated that waist circumference ( P = 0.01), sex ( P = 0.01), and percent body fat ( P = 0.05) were significant determinants of PDIFF. Molecular characterization of predifferentiated cultured cells was performed by real-time PCR measurements of glucocorticoid receptor-α (GRα), insulin-like growth factor I receptor (IGF-IR), peroxisome proliferator-activated receptor-γ (PPARγ), enhancer-binding protein GATA-3, CCAAT/enhancer-binding protein-α undifferentiated protein (CUP/AP-2α), and endothelial cell-specific marker 2 (ECSM2). The mRNA concentrations of GRα correlated with PDIFF ( r = 0.29, P = 0.03), but the others did not (IGF-IR, r = 0.003, P = 1.0; PPARγ, r = −0.1, P = 0.5; GATA-3, r = 0.02, P = 0.9; CUP/AP-2α, r = −0.2, P = 0.1; ECSM2, r = 0.04, P = 0.7). Contrary to our hypothesis, the results may indicate a blunted in vitro differentiation potential of preadipocytes in centrally obese individuals. The lower differentiation potential of preadipocytes in the obese subjects might be due, at least partly, to decreased glucocorticoid receptor expression.

Publisher

American Physiological Society

Subject

Physiology (medical),Physiology,Endocrinology, Diabetes and Metabolism

Cited by 79 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3