A cardiac-like sodium current in motor neurons of a jellyfish

Abstract

1. Whole cell voltage-clamp recordings from isolated swimming motor neurons (SMNs) reveal a rapidly activating and inactivating sodium current. 2. Permeability ratios of PLi/PNa = 0.941 and P(guanidinium)/PNa = 0.124 were measured for the mediating channel, which was impermeable to rubidium. 3. The conductance/voltage and steady state inactivation curves are shifted in a depolarizing direction by approximately 45 mV relative to most neuronal sodium currents in higher animals. 4. Activation could be fitted with two exponents and maximal current peaked at 0.74 +/- 0.06 ms (mean +/- SD). 5. Inactivation could be fitted with fast (Tau 1 = 1.91 +/- 0.07 ms at +10 mV) and slow (Tau 2 = 11.65 +/- 0.55 ms at +10 mV) exponents. 6. Half-recovery from inactivation occurred slowly (52.6 +/- 2.9 ms). 7. A second class of identifiable neurons, "B" neurons, possesses a distinctly different population of sodium channels. they showed different inactivation kinetics and far more rapid recovery from inactivation (half-recovery < 5 ms). 8. We conclude that there was physiological diversification of sodium channels early in metazoan evolution and that there has been considerable cell-specific selection of channel properties.