Affiliation:
1. Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, Nevada
Abstract
Purines induce transient contraction and prolonged relaxation of detrusor muscles. Transient contraction is likely due to activation of inward currents in smooth muscle cells, and prolonged relaxation may be due to activation of small-conductance Ca2+-activated K+ (SK) channels via P2Y1 receptors expressed by detrusor PDGF receptor (PDGFR)α+ cells. We investigated whether other subtypes of P2Y receptors are involved in the activation of SK channels in PDGFRα+ cells of detrusor muscles. Quantitative analysis of transcripts revealed that P2ry2, P2ry4, and P2ry14 are expressed in PDGFRα+ cells of P2ry1-deficient/enhanced green fluorescent protein ( P2ry1−/−/eGFP) mice at similar levels as in wild-type mice. UTP, a P2Y2/P2Y4 agonist, activated large outward currents in detrusor PDGFRα+ cells. SK channel blockers and an inhibitor of phospholipase C completely abolished currents activated by UTP. In contrast, UTP activated nonselective cation currents in smooth muscle cells. Under current-clamp (current = 0), UTP induced significant hyperpolarization of PDGFRα+ cells. MRS2500, a selective P2Y1 antagonist, did not affect UTP-activated outward currents in PDGFRα+ cells from wild-type mice, and activation of outward currents by UTP was retained in P2ry1−/−/eGFP mice. As a negative control, we tested the effect of MRS2693, a selective P2Y6 agonist. This compound did not activate outward currents in PDGFRα+ cells, and currents activated by UTP were unaffected by MRS2578, a selective P2Y6 antagonist. The nonselective P2Y receptor blocker suramin inhibited UTP-activated outward currents in PDGFRα+ cells. Our data demonstrate that P2Y2 and/or P2Y4 receptors function, in addition to P2Y1 receptors, in activating SK currents in PDGFRα+ cells and possibly in mediating purinergic relaxation responses in detrusor muscles.
Funder
HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Publisher
American Physiological Society
Cited by
13 articles.
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