Morphologic and morphometric study on microvasculature of developing mouse kidneys

Author:

Zhang Jie1,Cong Jing12,Yang Jie1,Thomsen Jesper Skovhus3,Andreasen Arne3ORCID,Chang Shi-Jie4,Wang Kai-Yue1,Gu Ling1,Zhai Xiao-Yue1

Affiliation:

1. Department of Histology and Embryology, Basic Medicine College, China Medical University, Shenyang, China

2. Department of Histology and Embryology, Shenyang Medical College, Shenyang, China

3. Department of Biomedicine-Anatomy, Aarhus University, Aarhus, Denmark

4. Department of Biomedical Engineering, College of Fundamental Science, China Medical University, Shenyang, China

Abstract

A proper morphogenesis of the renal microvasculature is crucial not only for fulfilling the renal function but also to slow down the progression of chronic kidney disease in adulthood. However, the current description of the developing microvasculature is incomplete. The present study investigated the morphogenesis and volume densities of the renal microvasculature using computer-assisted tubular tracing, immunohistochemistry for CD34, and unbiased stereology. The earliest glomerular capillaries were observed at the lower cleft of the S-shaped nephrons, as simple loops connecting the afferent and efferent arterioles. In parallel with this, the peritubular capillaries were established. Noticeably, from early nephrogenesis on, the efferent arterioles of the early-formed glomeruli ran in close proximity to their own thick ascending limbs. In addition, the ascending vasa recta arising from the arcuate or interlobular veins also ran in close proximity to the thick descending limb. Thus, the tubules and vessels formed the typical countercurrent relation in the medulla. No loop bends were observed between descending and ascending vasa recta. The volume density of the cortical and medullary peritubular capillary increased 3.3- and 2.6-fold, respectively, from 2.34 (0.13) and 7.03 (0.09)% [means (SD)] at embryonic day 14.5 (E14.5) to 7.71 (0.44) and 18.27 (1.17)% at postnatal day 40 (P40). In contrast, the volume density of glomeruli changed only slightly during kidney development, from 4.61 (0.47)% at E14.5 to 6.07 (0.2)% at P7 to 4.19 (0.47)% at P40. These results reflect that the growth and formation of the renal microvasculature closely correspond to functional development of the tubules.

Funder

National Natural Science Foundation of China (NSFC)

Publisher

American Physiological Society

Subject

Physiology

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