A novel ex vivo protocol to mimic human walking gait: implications for Duchenne muscular dystrophy-Reference-Cited by-同舟云学术

A novel ex vivo protocol to mimic human walking gait: implications for Duchenne muscular dystrophy

Published:2020-10-01 Issue:4 Volume:129 Page:779-791
ISSN:8750-7587
Container-title:Journal of Applied Physiology
language:en
Short-container-title:Journal of Applied Physiology

Author:

Bukovec Katherine E.¹,Hu Xiao²,Borkowski Matthew³,Jeffery Duane³,Blemker Silvia S.²⁴⁵,Grange Robert W.¹

Affiliation:

1. Department of Human Nutrition, Foods, and Exercise and Metabolism Core, Virginia Tech, Blacksburg, Virginia

2. Department of Biomedical Engineering, University of Virginia, Charlottesville, Virginia

3. Aurora Scientific, Inc., Aurora, Ontario, Canada

4. Department of Orthopedic Surgery, University of Virginia, Charlottesville, Virginia

5. Department of Mechanical and Aerospace Engineering, University of Virginia, Charlottesville, Virginia

Abstract

A novel ex vivo mouse soleus protocol that mimics scaled length change and excitation profiles predicted by a mathematical model of human soleus during gait is presented. A custom stimulator was developed that enabled an innovative muscle stimulation technique to modulate voltage to closely match the excitation pattern of human soleus during gait. This ex vivo protocol provides assessment of simulated human movement in mouse muscle, including components of eccentric contractions.

Funder

NIH

Publisher

American Physiological Society

Subject

Physiology (medical),Physiology

Link

https://journals.physiology.org/doi/pdf/10.1152/japplphysiol.00002.2020

Reference63 articles.

1. Fibre operating lengths of human lower limb muscles during walking

2. A Model of the Lower Limb for Analysis of Human Movement

3. Transgenic overexpression of γ-cytoplasmic actin protects against eccentric contraction-induced force loss in mdx mice

4. Regenerated soleus muscle shows reduced creatine kinase efflux after contractile activity in vitro

Cited by 3 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. The impacts of muscle-specific force-velocity properties on predictions of mouse muscle function during locomotion;Frontiers in Bioengineering and Biotechnology;2024-07-23

2. Perturbing the muscle work loop paradigm to unravel the neuromechanics of unsteady locomotion;Journal of Experimental Biology;2023-04-01

3. A novel murine muscle loading model to investigate Achilles musculotendinous adaptation;Journal of Applied Physiology;2021-04-01